Identification of crystallin family proteins in vitreous body in rat endotoxin-induced uveitis: Involvement of crystallin truncation in uveitis pathogenesis
Identification of crystallin family proteins in vitreous body in rat endotoxin-induced uveitis: Involvement of crystallin truncation in uveitis pathogenesis
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DOI：
10.1002/pmic.200500779
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发表时间：
2006-06-01
期刊：
影响因子：
3.4
通讯作者：
Chung, Hun-Taeg
中科院分区：
文献类型：
--
作者：
Bahk, Song-Chul;Lee, Sook-Hee;Chung, Hun-Taeg
内毒素诱导的葡萄膜炎（EIU）是一种急性眼部炎症的动物模型。为了表征 EIU 的机制，我们使用 2-DE 和微型 LC/LC-MS/MS 分析了 EIU 大鼠和正常大鼠玻璃体中蛋白质的浸润。在大鼠玻璃体中发现了二十个斑点。其中十八个斑点是晶状体蛋白家族的成员。正常玻璃体中以截短形式的 β A4- 和 β B2- 晶状体蛋白为主，但在注射脂多糖的 EIU 大鼠中存在完整形式的晶状体蛋白。这些结果表明，晶状体蛋白家族蛋白是参与葡萄膜炎玻璃体的主要蛋白组，β-晶状体蛋白的 C 端截短可能在 EIU 相关疾病进展中发挥作用。
Endotoxin-induced uveitis (EIU) is an animal model of acute ocular inflammation. To characterize the mechanism of EIU, we analyzed the infiltration of proteins in the vitreous bodies of rats with EIU and normal rats using 2-DE and micro LC/LC-MS/MS. Twenty spots were identified in vitreous bodies of rats. Eighteen of these spots were members of the crystallin family. The truncated form of beta A4- and beta B2-crystallin were predominant in normal vitreous bodies, but there were intact form of crystallins in lipopolysaccharide-injected rats with EIU. These results suggest that crystallin family proteins are the major group of proteins involved in uveitic vitreous and that C-terminal truncation of beta-crystallins may play a role in EIU-related disease progression.