MORPHOLOGICAL AND QUANTITATIVE-ANALYSIS OF SPERMATOGONIA IN MOUSE TESTES USING WHOLE MOUNTED SEMINIFEROUS TUBULES .1. NORMAL TESTES
MORPHOLOGICAL AND QUANTITATIVE-ANALYSIS OF SPERMATOGONIA IN MOUSE TESTES USING WHOLE MOUNTED SEMINIFEROUS TUBULES .1. NORMAL TESTES
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DOI：
10.1002/ar.1091920406
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发表时间：
1978-01-01
影响因子：
--
通讯作者：
OAKBERG, EF
中科院分区：
文献类型：
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作者：
HUCKINS, C;OAKBERG, EF
用全装精管对10只正常成年小鼠的精原细胞群进行了分析。未分化的A型精原细胞和6代分化的A型精原细胞在全株上清晰可见。对这些细胞类型的描述和定量表明，它们的行为方式与大鼠中的对应细胞基本相同。单个未分化的A细胞被归类为A型干细胞精原细胞。它们分布在整个精原上皮中，通过周期性的有丝分裂，维持了它们的存量并提供了最终分化的细胞。虽然最初类似于a型精原细胞，但注定分化的后代被归类为Aal型精原细胞，因为它们通过细胞质桥连接，并且它们通常经历1次或更多的同步有丝分裂形成短链排列的细胞。最终，Aal细胞不再分裂，细胞似乎逐渐获得了Al精原细胞的典型形态特征。这些继续按照既定的模式进行区分。以这种方式循环产生的Al细胞群显然可以确保精原细胞的持续供应以供分化。
The spermatogonial populations in 10 normal adult mice were analyzed using whole mounted seminiferous tubules. The undifferentiated A spermatogonia, as well as the 6 generations of differentiating spermatogonia, were clearly identifiable on whole mounts. Description plus quantitation of these cell types revealed that they behaved in essentially the same manner as their counterparts in the rat. Single undifferentiated A cells were classified as type As stem cell spermatogonia. They were distributed throughout the seminiferous epithelium, and by periodic mitoses, maintained their stock and furnished cells which would eventually differentiate. Although initially resembling the As spermatogonia, the progeny which were destined to differentiate were classified as type Aal spermatogonia because they were linked by cytoplasmic bridges, and because they usually underwent 1 or more synchronous mitotic divisions to form short chains of aligned cells. Ultimately, division of Aal cells were no longer seen, and the cells appeared to gradually acquire the typical morphological characteristics of Al spermatogonia. These continued to differentiate according to the well-established pattern. The cyclic production of cohorts of Al cells in this manner would apparently ensure a continual supply of spermatogonia for differentiation.