Bacterial diversity in intestinal mucosa of antibiotic-associated diarrhea mice
Bacterial diversity in intestinal mucosa of antibiotic-associated diarrhea mice
复制标题
抗生素相关性腹泻小鼠肠粘膜细菌多样性
DOI：
10.1007/s13205-019-1967-2
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发表时间：
2019-12-01
期刊：
影响因子：
2.8
通讯作者：
Tan, Zhoujin
中科院分区：
文献类型：
--
作者：
Xie, Guozhen;Tan, Kai;Tan, Zhoujin
To probe into the mechanism of antibiotic-associated diarrhea (AAD), the bacterial diversity and composition in the intestinal mucosa of AAD mice were investigated. Twelve specific pathogen-free Kunming mice were divided into control group and model group. The mouse model of AAD was established by gavaging with antibiotics (mixture of gentamycin sulfate and cefradine) at a total dose of 23.33 ml kg−1day−1for 5 days continuously, twice a day. The mice in the control group were given with an equal amount of sterile water. Then, the intestinal mucosa DNA was extracted for 16S rRNA gene sequence analysis by high-throughput sequencing. The results showed that the alpha diversity of the two groups did not differ significantly from each other, while the composition of intestinal mucosa bacteria differed dramatically between the two groups. The model group showed a higher abundance of Proteobacteria and Actinobacteria. More importantly,Lactobacilluswas significantly less abundant (p= 0.000), whileEnterococcuswas significantly more abundant (p= 0.019) in the model group than in the control group. Furthermore, antibiotic treatment increased the abundance ofCitrobacter,Stenotrophomonas, andGlutamicibacter,whereas antibiotics decreased the abundance ofMycoplasmaandHelicobacter. In addition, 6 and 11 unique genera were found in the control group and model group, respectively. The combination of gentamycin sulfate and cefradine changed the intestinal mucosa bacterial composition, reduced colonization resistance and damaged the intestinal mucosal barrier by reducing the abundance ofLactobacillus.
