Adipose Tissue as a Useful Material for the Grafting of Tumorigenic Cells and Juvenile Tissues in Mice
Adipose Tissue as a Useful Material for the Grafting of Tumorigenic Cells and Juvenile Tissues in Mice
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DOI：
10.21926/obm.transplant.2104155
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发表时间：
2021-03
期刊：
影响因子：
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通讯作者：
Masahiro Sato;Y. Kiyokawa;E. Inada;Eri Akasaka;Satoshi Watanabe;I. Saitoh
中科院分区：
文献类型：
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作者：
Masahiro Sato;Y. Kiyokawa;E. Inada;Eri Akasaka;Satoshi Watanabe;I. Saitoh
虽然衰老过程扩大了小鼠体内脂肪组织的居住范围，而且由于脂肪组织与雌性生殖系统密切相关，但当生殖器官（包括卵巢、输卵管和子宫的一部分）被拉出暴露在背部皮肤上时，它很容易在麻醉下手术暴露。本研究旨在考虑脂肪组织作为操作靶点的适用性，特别是细胞或小尺寸组织切片的移植，因为它易于操作。随后，在解剖显微镜下使用呼吸控制的微移液管给组织注射1-2µL的台泮蓝，以评估脂肪组织作为合适移植材料的潜力。我们观察到注射的染料在注射B16小鼠黑色素瘤细胞和P19胚胎癌细胞后至少在注射部位停留了一天。注射后1个半月的检查结果显示，卵巢、输卵管或子宫周围的实体瘤的发生率为100%。当对1 / 4的幼年胰腺（15天）进行移植手术时，生成了一个形态形态正常的胰腺（包括胰岛素合成细胞的形成），在注射一个月后的检查中观察到，从而成功地验证了我们的脂肪组织操纵方法，并将这项新技术命名为“脂肪内细胞和组织导入”。
Although the aging process expands the adipose tissue habitation in mice and due to its close association with the female reproductive system, it can be easily exposed surgically under anesthesia when reproductive organs (including ovary, oviduct, and part of the uterus) are pulled and exposed onto the dorsal skin. This study aimed to consider the suitability of adipose tissue as a target for manipulation, particularly for the grafting of cells or small-sized tissue sections due to its ease of handling. Subsequently, 1-2 µL trypan blue injections were administered to the tissues using a breath-controlled micropipette under a dissecting microscope for evaluating the adipose tissue’s potential as a suitable grafting material. It was observed that the injected dye remained at the injection site for at least one day after injecting B16 mouse melanoma and P19 embryonal carcinoma cells. It resulted in the generation of solid tumors surrounding the ovary, oviduct, or uterus with 100% efficiency, as reported by an inspection one and a half months after the injections. When the grafting procedure was carried out for one-fourth of the juvenile pancreas (aged 15 days), an enlarged pancreas with normal morphological configuration (including the formation of insulin-synthesizing cells) was generated, which was observed by an inspection one month after the injections, thus successfully validating our approach for adipose tissue manipulation and furthermore, naming this novel technology as “intra-adipose introduction of cells and tissues”.