CHARACTERIZATION OF POPULATIONS OF RAPID-CYCLING BRASSICA-RAPA L SELECTED FOR DIFFERENTIAL WATERLOGGING TOLERANCE
CHARACTERIZATION OF POPULATIONS OF RAPID-CYCLING BRASSICA-RAPA L SELECTED FOR DIFFERENTIAL WATERLOGGING TOLERANCE
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DOI：
10.1093/jxb/45.3.385
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发表时间：
1994-03-01
影响因子：
6.9
通讯作者：
MUSGRAVE, ME
中科院分区：
文献类型：
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作者：
DAUGHERTY, CJ;MUSGRAVE, ME
通过用涝胁迫挑战异质植物群体（快速循环甘蓝），我们选择了对根区缺氧反应不同的植物。根据淹水后叶子的颜色，这些个体被分为“耐受”和“敏感”群体，然后进行大规模授粉并重新选择七代以上，以产生所描述的稳定群体。为了评估所选群体对根区缺氧的反应，植物在正常浇水条件下发芽后生长1周，然后承受长达8天的涝害胁迫。在对照条件下，耐受群体和敏感群体之间的任何研究参数均未发现差异。当植物被淹水时，耐受群体中的叶绿素浓度显着高于敏感群体中的浓度。在根和芽干物质累积中也发现了类似的胁迫特异性差异。随着土壤氧化还原值（以及有效氧）的减少，浸水植物叶子中可溶性碳水化合物和淀粉的含量增加。早在敏感植物浸水后 12 小时就注意到可溶性碳水化合物的变化，并且在浸水后 24 小时该群体的淀粉浓度显着升高。淹水条件下，乙醇脱氢酶（ADH）和丙酮酸脱羧酶（PDC）活性升高，磷酸葡萄糖变位酶和苹果酸脱氢酶活性降低，苹果酸酶和葡萄糖6-磷酸脱氢酶活性没有变化。淹水18 h和48 h后，敏感种群ADH和PDC活性分别超过耐受种群。结果表明，种群对涝灾反应的胁迫特异性差异可以通过反复选择来实现。
By challenging a heterogenous population of plants (rapid-cycling Brassica rapa L.) with waterlogging stress, we selected plants which differed in their response to root zone hypoxia. These individuals were placed into 'tolerant' and 'sensitive' populations based on foliage colour after waterlogging and were then mass-pollinated and re-selected over seven generations to produce the stable populations described. to assess responses to root zone hypoxia in the selected populations, plants were grown for 1 week after germination under normal watering conditions and then subjected to waterlogging stress for up to 8 d. Under control conditions, no differences were found between the tolerant and sensitive populations in any of the parameters studied. Chlorophyll concentrations in the tolerant population were significantly greater than the concentrations in the sensitive population when plants had been waterlogged. A similar stress-specific difference was found in root and shoot dry matter accrual. As soil redox values (and hence, available oxygen) decreased, an increase in soluble carbohydrates and starch occurred in the leaves of waterlogged plants. Changes in soluble carbohydrates were noted as early as 12 h after waterlogging in the sensitive plants, and starch concentrations were significantly higher for this population 24 h after waterlogging. Under waterlogged conditions, activities of alcohol dehydrogenase (ADH) and pyruvate decarboxylase (PDC) increased, phosphoglucomutase and malate dehydrogenase decreased, and malic enzyme and glucose 6-phosphate dehydrogenase did not change. The sensitive population exceeded the tolerant population in activities of ADH and PDC after 18 and 48 h of waterlogging, respectively. The results demonstrate that stress-specific differences in population responses to waterlogging can be achieved through recurrent selection.