Increased cytotoxicity of ricin in a putative Golgi-defective mutant of Chinese hamster ovary cell.
Increased cytotoxicity of ricin in a putative Golgi-defective mutant of Chinese hamster ovary cell.
复制标题
在中国仓鼠卵巢细胞的假定高尔基体缺陷突变体中，蓖麻毒素的细胞毒性增加。
DOI：
10.1016/0014-4827(90)90137-y
复制
发表时间：
1990
影响因子：
3.7
通讯作者：
Wu,HC
中科院分区：
文献类型：
--
作者：
Yoshida,T;Chen,CH;Zhang,MS;Wu,HC
我们研究了蓖麻毒素对中国仓鼠卵巢(CHO)细胞系耐莫能菌素突变体(monr-31)的细胞毒性作用。与亲本CHO细胞相比，在monr-31突变细胞中，蓖麻毒素的细胞毒作用增强。在野生型CHO细胞中，NH4Cl、巴菲尔霉素A1或黑素可增强蓖麻毒素的细胞毒作用。在monr-31突变细胞中，这些化合物对蓖麻毒素细胞毒性的增强作用大大降低。Brefeldin A(BFA)破坏高尔基体的结构，抑制蓖麻毒素对CHO和MonR-31细胞的细胞毒作用。我们还研究了糖基化抑制剂和去除蓖麻毒素中的高甘露糖寡糖链对MonR-31细胞的蓖麻毒素超敏反应的影响。MonR-31细胞对蓖麻毒素的超敏反应显然不是由于CHO和MonR-31细胞之间的糖基化或靶细胞对蓖麻毒素低聚糖的加工过程的不同造成的。低浓度(10 NM)的尼日利亚毒素对白喉毒素的细胞毒性无影响，但可增强蓖麻毒素的细胞毒性，但抑制莫德霉素的细胞毒性。我们的结果表明，在蓖麻毒素和莫德霉素中毒CHO细胞的过程中，重要的一步(S)发生在高尔基体。
We have studied the cytotoxicity of ricin in a monensin-resistant mutant (MonR-31) of Chinese hamster ovary (CHO) cell line which is presumably altered in Golgi functions/structures. The cytotoxicity of ricin was increased in MonR-31 mutant cells compared with that in its parental CHO cells. In wild-type CHO cells, the cytotoxicity of ricin was enhanced by NH4Cl, bafilomycin A1, or nigericin. The enhancement of ricin cytotoxicity by these compounds was greatly reduced in MonR-31 mutant cells. Brefeldin A (BFA), which disrupts the structure of the Golgi apparatus, inhibits the cytotoxicity of ricin in both CHO and MonR-31 cells. We have also examined the effects of glycosylation inhibitors and the removal of high mannose oligosaccharide chains in ricin on the ricin hypersensitivity in MonR-31 cells. The hypersensitivity of MonR-31 cells to ricin is apparently not due to any difference in glycosylation between CHO and MonR-31 cells or in the processing of oligosaccharides on ricin by the target cells. Nigericin at low concentration (10 nM), which has no effect on the cytotoxicity of diphtheria toxin, enhances the ricin cytotoxicity, but inhibits the modeccin cytotoxicity. Our results suggest that important step(s) in the intoxication process of CHO cells by ricin and modeccin take place in the Golgi region.