Comparison of Long Non-Coding RNA Expression Profiles of Cattle and Buffalo Differing in Muscle Characteristics
Comparison of Long Non-Coding RNA Expression Profiles of Cattle and Buffalo Differing in Muscle Characteristics
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肌肉特征不同的牛和水牛的长非编码 RNA 表达谱的比较
DOI：
10.3389/fgene.2020.00098
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发表时间：
2020-02-26
影响因子：
3.7
通讯作者：
Liu, Qingyou
中科院分区：
文献类型：
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作者：
Li, Hui;Huang, Kongwei;Liu, Qingyou
水牛肉具有良好的质量特征，因为它含有有利于心血管系统的“薄嫩”。然而，长链非编码RNA （lncRNA）调控肉质差异的机制尚不清楚。化学物理参数表明，水牛的肌肉质量与牛相当，但在剪切力和肌纤维结构上存在显著差异。然后，我们研究了水牛和牛骨骼肌的lncRNA表达谱，首次深入了解了它们在水牛肌肉形成中的潜在作用。在这里，我们分析了牛和水牛骨骼肌组织中lncRNA的表达，在比较水牛和牛的肌肉组织时，检测到16236个候选lncRNA，其中865个lncRNA上调，1296个lncRNA下调。我们构建了共表达和ceRNA网络，发现lncRNA MSTRG.48330.7、MSTRG.30030.4和MSTRG.203788.46可能是含有miR-1/206和miR-133a潜在结合位点的竞争性内源性RNA （ceRNA）。组织表达分析显示，MSTRG.48330.7、MSTRG.30030.4和MSTRG.203788.46在肌肉组织中高特异性表达。本研究可作为起点研究水牛肌生成过程中几种lncrna所起作用的参考工具。
Buffalo meat consist good qualitative characteristics as it contains "thined tender" which is favorable for cardavascular system. However, the regulatory mechanisms of long non-coding RNA (lncRNA), differences in meat quality are not well known. The chemical-physical parameters revealed the muscle quality of buffalo that can be equivalent of cattle, but there are significant differences in shearing force and muscle fiber structure. Then, we examined lncRNA expression profiles of buffalo and cattle skeletal muscle that provide first insights into their potential roles in buffalo myogenesis. Here, we profiled the expression of lncRNA in cattle and buffalo skeletal muscle tissues, and 16,236 lncRNA candidates were detected with 865 up-regulated lncRNAs and 1,296 down-regulated lncRNAs when comparing buffalo to cattle muscle tissue. We constructed coexpression and ceRNA networks, and found lncRNA MSTRG.48330.7, MSTRG.30030.4, and MSTRG.203788.46 could be as competitive endogenous RNA (ceRNA) containing potential binding sites for miR-1/206 and miR-133a. Tissue expression analysis showed that MSTRG.48330.7, MSTRG.30030.4, and MSTRG.203788.46 were highly and specifically expressed in muscle tissue. Present study may be used as a reference tool for starting point investigations into the roles played by several of those lncRNAs during buffalo myogenesis.