G-rich, a Drosophila selenoprotein, is a Golgi-resident type III membrane protein
G-rich, a Drosophila selenoprotein, is a Golgi-resident type III membrane protein
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G-rich 是一种果蝇硒蛋白，是高尔基体驻留的 III 型膜蛋白
DOI：
10.1016/j.bbrc.2006.07.203
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发表时间：
2006-10-06
影响因子：
3.1
通讯作者：
Lee, Byeong Jae
中科院分区：
文献类型：
--
作者：
Chen, Chang Lan;Shim, Myoung Sup;Lee, Byeong Jae
G-rich is a Drosophila melanogaster selenoprotein, which is a homologue of human and mouse SelK. Subcellular localization analysis using GFP-tagged G-rich showed that G-rich was localized in the Golgi apparatus. The fusion protein was co-localized with the Golgi marker proteins but not with an endoplasmic reticulum (ER) marker protein in Drosophila SL2 cells. Bioinformatic analysis of G-rich suggests that this protein is either type II or type III transmembrane protein. To determine the type of transmembrane protein experimentally, GFP-G-rich in which GFP was tagged at the N-terminus of G-rich, or G-rich-GFP in which GFP was tagged at the C-terminus of G-rich, were expressed in SL2 cells. The tagged proteins were then digested with trypsin, and analyzed by Western blot analysis. The results showed that the C-terminus of the G-rich protein was exposed to the cytoplasm indicating it is a type III microsomal membrane protein. G-rich is the first selenoprotein identified in the Golgi apparatus. Published by Elsevier Inc.
