Demonstration and Analysis of the Suction Effect for Pumping Lymph from Tissue Beds at Subatmospheric Pressure
Demonstration and Analysis of the Suction Effect for Pumping Lymph from Tissue Beds at Subatmospheric Pressure
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DOI：
10.1038/s41598-017-11599-x
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发表时间：
2017-09-21
影响因子：
4.6
通讯作者：
Moore, James E., Jr.
中科院分区：
文献类型：
--
作者：
Jamalian, Samira;Jafarnejad, Mohammad;Moore, James E., Jr.
许多组织在正常生理条件下表现出低于大气压的间质压力。淋巴系统抵抗总体压力梯度从这些组织中提取液体的机制尚不清楚。我们通过将收缩功能和压力产生的实验测量与先前验证的数学模型相结合来解决这个重要的生理问题。我们为收集淋巴管中存在“吸入压力”提供了明确的证据，这表现为收缩后入口阀下游压力的短暂下降。该吸力打开入口阀，并且需要在上游压力较低的情况下进行填充。这种抽吸需要正跨壁压力，提供重新打开容器所需的能量。或者，当跨壁压力为负时，外部血管栓系可以起到相同的作用。吸力向上游传递，使液体通过最初的淋巴管被吸入。由于抽吸在液体进入淋巴管中起着重要作用，并且受到间质压力的影响，因此我们的研究结果将这种现象作为淋巴水肿及其治疗研究中需要考虑的另一个重要因素。
Many tissues exhibit subatmospheric interstitial pressures under normal physiologic conditions. The mechanisms by which the lymphatic system extracts fluid from these tissues against the overall pressure gradient are unknown. We address this important physiologic issue by combining experimental measurements of contractile function and pressure generation with a previously validated mathematical model. We provide definitive evidence for the existence of 'suction pressure' in collecting lymphatic vessels, which manifests as a transient drop in pressure downstream of the inlet valve following contraction. This suction opens the inlet valve and is required for filling in the presence of low upstream pressure. Positive transmural pressure is required for this suction, providing the energy required to reopen the vessel. Alternatively, external vessel tethering can serve the same purpose when the transmural pressure is negative. Suction is transmitted upstream, allowing fluid to be drawn in through initial lymphatics. Because suction plays a major role in fluid entry to the lymphatics and is affected by interstitial pressure, our results introduce the phenomenon as another important factor to consider in the study of lymphoedema and its treatment.