Cell differentiation and morphogenesis in the colony-forming choanoflagellate Salpingoeca rosetta
Cell differentiation and morphogenesis in the colony-forming choanoflagellate Salpingoeca rosetta
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DOI：
10.1016/j.ydbio.2011.06.003
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发表时间：
2011-09-01
影响因子：
2.7
通讯作者：
King, Nicole
中科院分区：
文献类型：
--
作者：
Dayel, Mark J.;Alegado, Rosanna A.;King, Nicole
据推测，动物的发育是从动物的单细胞和群体祖先中预先存在的调节细胞分化的机制进化而来的。虽然不能直接研究动物的祖先，但可以通过比较动物与它们最近的近亲--领鞭毛虫--来了解它们的细胞生物学。我们在这里报告的生活史，细胞分化和细胞间的相互作用，在集落形成choanoflagellate Salpingoeca rosetta。在不同的环境线索，S。Rosetta分化成至少五种不同的细胞类型，包括三种单独的细胞类型（慢泳者、快泳者和囊细胞）和两种群体形式（roplum和chains）。电子显微镜显示，集落内的细胞通过精细的细胞间桥、共享的细胞外基质和丝状伪足的组合而保持在一起。此外，我们还发现碳水化合物结合蛋白小麦胚芽凝集素特异性地染色菌落和它们形成的缓慢游泳者，表明分子分化先于多细胞发育。总之，这些结果有助于建立S。Rosetta作为研究领鞭毛虫简单多细胞性的模型系统，并为研究动物多细胞性的起源和发育提供了实验框架。(C)2011 Elsevier Inc. All rights reserved.
It has been posited that animal development evolved from pre-existing mechanisms for regulating cell differentiation in the single celled and colonial ancestors of animals. Although the progenitors of animals cannot be studied directly, insights into their cell biology may be gleaned from comparisons between animals and their closest living relatives, the choanoflagellates. We report here on the life history, cell differentiation and intercellular interactions in the colony-forming choanoflagellate Salpingoeca rosetta. In response to diverse environmental cues, S. rosetta differentiates into at least five distinct cell types, including three solitary cell types (slow swimmers, fast swimmers, and thecate cells) and two colonial forms (rosettes and chains). Electron microscopy reveals that cells within colonies are held together by a combination of fine intercellular bridges, a shared extracellular matrix, and filopodia. In addition, we have discovered that the carbohydrate-binding protein wheat germ agglutinin specifically stains colonies and the slow swimmers from which they form, showing that molecular differentiation precedes multicellular development. Together, these results help establish S. rosetta as a model system for studying simple multicellularity in choanoflagellates and provide an experimental framework for investigating the origin of animal multicellularity and development. (C) 2011 Elsevier Inc. All rights reserved.