Carbon budgets in batch and continuous cultures: How can we understand natural physiology of marine phytoplankton?
Carbon budgets in batch and continuous cultures: How can we understand natural physiology of marine phytoplankton?
复制标题
分批和连续培养中的碳预算：我们如何理解海洋浮游植物的自然生理学？
DOI：
10.1093/plankt/2.3.213
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发表时间：
1980
影响因子：
2.1
通讯作者：
Andrew C. Frake
中科院分区：
文献类型：
--
作者：
J. Sharp;P. Underhill;Andrew C. Frake
对海链藻碳同化过程中呼吸损失和有机损失的监测表明，在连续培养中，总碳损失很小（约5%）。虽然批培养和连续培养细胞化学之间的相关性不太理想，但批培养指数期细胞显示出与连续培养相似的碳代谢。在这两种情况下，生长速率都超过1.5次分裂d-1，分批指数期的总碳损失与连续培养中的相似（<10%）。从C-14吸收和颗粒碳的增长率的估计值匹配的细胞数量的变化，在分批培养和连续培养的稀释率。在分批培养中，在开始培养后立即和在稳定期，来自呼吸、有机排泄和细胞降解的碳损失很大（>50%）。为了了解自然界中浮游植物的生理学，有必要找出生长速度和种群密度对碳损失的影响，并确定稳态条件是否真正适用于海洋。
Monitoring of respiratory and organic losses from carbon assimilation for the diatomThalassiosira pseudonanashows very slight (about 5%) total carbon losses in continuous culture. Although correlation between batch and continuous culture cellular chemistry was less than ideal, batch culture exponential phase cells showed carbon metabolism similar to that for the continuous culture. In both cases, growth rates were in excess of 1.5 divisions d−1and total carbon losses in the batch exponential phase were similar (<10%) to those in the continuous culture. Estimates of growth rates from C-14 uptake and particulate carbon matched those from changes in cell numbers in the batch culture and that from the dilution rate in the continuous culture. In batch culture, immediately after starting the culture and in stationary phase, carbon losses from respiration, organic excretion, and cellular degradation were large (>50%). To understand phytoplankton physiology in nature, it is necessary to find what effects growth rate and population density have on carbon losses and to ascertain whether or not steady state conditions really pertain to the ocean.