Differentiation Ability of Dental Pulp Cells of Deciduous Teeth under Hypoxia
Differentiation Ability of Dental Pulp Cells of Deciduous Teeth under Hypoxia
复制标题
缺氧条件下乳牙牙髓细胞的分化能力
DOI：
10.11223/jarde.15.65
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Kenji Arita
中科院分区：
文献类型：
--
作者：
Saki Kawai;Kyoko Harada;Sho Aoki;Yukari Shinonaga;Yoko Abe;Kenji Arita
干细胞是近年来再生医学研究的热点，牙髓细胞被认为是干细胞的来源之一。乳牙牙髓细胞的增殖和分化明显大于恒牙牙髓细胞。一些报道表明，缺氧可上调干细胞的增殖和分化。本研究探讨了缺氧对人乳牙牙髓细胞的影响。2% O2缺氧2 d对乳牙牙髓细胞增殖无影响。但增加了缺氧诱导因子-1-1α、Oct3/4、Sox2、C-Myc的基因表达，促进了成脂分化。结果表明，缺氧可增强乳牙牙髓细胞的分化能力。
Stem cells have been a recent focus of regenerative medicine research, and dental pulp cells are considered as a source of stem cells. Cell proliferation and differentiation of dental pulp cells in deciduous teeth are greater than those of dental pulp cells in permanent teeth. Some reports indicate that hypoxia upregulates the proliferation and differentiation of stem cells. In this study, we examined the effect of hypoxia on human dental pulp cells of deciduous teeth. Hypoxia at 2% O2 for 2 days did not affect cell proliferation of human dental pulp cells from deciduous teeth. However, it increased the gene expression of hypoxia inducible factor-1-1α, Oct3/4, Sox2, and C-Myc, and enhanced adipogenic differentiation. These results suggest that hypoxia enhances the differentiation ability of dental pulp cells in deciduous teeth.