ROTATIONAL RELAXATION OF 70S RIBOSOMES BY A DEPOLARIZATION METHOD USING TRIPLET PROBES
ROTATIONAL RELAXATION OF 70S RIBOSOMES BY A DEPOLARIZATION METHOD USING TRIPLET PROBES
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DOI：
10.1073/pnas.74.4.1407
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发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
POCHON, F
中科院分区：
文献类型：
--
作者：
LAVALETTE, D;AMAND, B;POCHON, F
在微秒时间尺度上的旋转松弛之后，利用共价结合标签的长寿命三重态的特性进行了去极化技术。描述了两种有效结合核糖体蛋白的三联体探针。测定了大肠杆菌70S核糖体的旋转相关时间。溶液中功能活性70S颗粒的平均水动力半径估计为147 .ANG。观察到相关时间的浓度依赖性，这可能是由于70S核糖体结合形成100S二聚体的结果。
Rotational relaxation on the microsecond time scale was followed by a depolarization technique using the properties of the long-lived triplet state of covalently bound labels. Two triplet probes, which efficiently bind to ribosomal proteins, are described. The rotational correlation time of 70S ribosomes of Escherichia coli was measured. The average hydrodynamic radius of the functionally active 70S particle in solution was estimated to 147 .ANG.. A comcentration dependence of the correlation time was observed, which may result from an association of the 70S ribosomes to form 100S dimers.