Hydroxyiminodisuccinic acid (HIDS): A novel biodegradable chelating ligand for the increase of iron bioavailability and arsenic phytoextraction.
Hydroxyiminodisuccinic acid (HIDS): A novel biodegradable chelating ligand for the increase of iron bioavailability and arsenic phytoextraction.
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DOI：
10.1016/j.chemosphere.2009.07.032
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发表时间：
2009-09
期刊：
影响因子：
8.8
通讯作者：
M. Rahman;H. Hasegawa;K. Kadohashi;T. Maki;K. Ueda
中科院分区：
文献类型：
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作者：
M. Rahman;H. Hasegawa;K. Kadohashi;T. Maki;K. Ueda
研究了可生物降解螯合配体对水培水稻幼苗吸收砷和铁的影响。被查即使生长液中含有足够的铁，水稻幼苗的生长逐渐下降，高达76%的溶液的pH值从7到11的增加。铁在中性或碱性条件下形成不溶性氢氧化铁络合物。螯合配体产生可溶性的“铁-配体复合物”，其有助于植物对铁的吸收。可生物降解螯合配体羟亚氨基二琥珀酸（HIDS）比乙二胺四乙酸（EDTA）、乙二胺二琥珀酸（EDDS）和亚氨基二琥珀酸（IDS）更能促进水稻幼苗对铁的吸收和生长。在营养液中添加0.5mM的EDDS、EDTA、HIDS和IDS，水稻幼苗的干生物量分别为79±20、87±6、116±15和63± 18 mg。当在营养液中加入0.25mM的EDDS、EDTA、HIDS和IDS时，水稻组织中的Fe浓度分别为117±15、82±8、167±25和118±22μmolg-1干重。加入螯合配体后，水稻组织中积累的铁大部分储存在根部。结果表明，HIDS是一种潜在的替代环境持久性EDTA的铁吸收和植物生长的增加。HIDS也增加了水稻根系对As的吸收，但并没有增加As从根向地上部的转运。本研究首次报道了HIDS作为一种有前途的螯合配体，用于提高Fe的生物利用度和As的植物提取。
The influence of biodegradable chelating ligands on arsenic and iron uptake by hydroponically grown rice seedlings (Oryza sativa L.) was investigated. Even though the growth solution contained sufficient Fe, the growth of rice seedlings gradually decreased up to 76% with the increase of pH of the solution from 7 to 11. Iron forms insoluble ferric hydroxide complexes at neutral or alkaline pH in oxic condition. Chelating ligands produce soluble ‘Fe–ligand complex’ which assist Fe uptake in plants. The biodegradable chelating ligand hydroxyiminodisuccinic acid (HIDS) was more efficient then those of ethylenediaminetetraacetic acid (EDTA), ethylenediaminedisuccinic acid (EDDS), and iminodisuccinic acid (IDS) in the increase of Fe uptake and growth of rice seedling. A total of 79±20, 87±6, 116±15, and 63±18mg dry biomass of rice seedlings were produced with the addition of 0.5mM of EDDS, EDTA, HIDS, and IDS in the nutrient solution, respectively. The Fe concentrations in rice tissues were 117±15, 82±8, 167±25, and 118±22μmolg−1dry weights when 0.25mM of EDDS, EDTA, HIDS, and IDS were added to the nutrient solution, respectively. Most of the Fe accumulated in rice tissues was stored in roots after the addition of chelating ligands in the solution. The results indicate that the HIDS would be a potential alternative to environmentally persistent EDTA for the increase of Fe uptake and plant growth. The HIDS also increased As uptake in rice root though its translocation from root to shoot was not augmented. This study reports HIDS for the first time as a promising chelating ligand for the enhancement of Fe bioavailability and As phytoextraction.