Expression and sub-cellular localization of the CCAAT/enhancer binding protein alpha in relation to postnatal development and malignancy of the prostate.
Expression and sub-cellular localization of the CCAAT/enhancer binding protein alpha in relation to postnatal development and malignancy of the prostate.
复制标题
CCAAT/增强子结合蛋白α的表达和亚细胞定位与产后发育和前列腺恶性肿瘤的关系。
DOI：
10.1002/pros.20779
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Ratnam,Manohar
中科院分区：
文献类型：
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作者：
Zhang,Juan;Wilkinson,JohnErby;Gonit,Mesfin;Keck,Rick;Selman,Steven;Ratnam,Manohar
背景C/EBPα通过对细胞周期调节蛋白的强烈抗增殖作用而成为终末分化的关键介导剂和肿瘤抑制剂。C/EBPα似乎也调节雄激素受体（AR）AR信号传导。有，是一个缺乏的信息表达和亚细胞定位的C/EBPα在正常小鼠和人类前列腺和在prostate cancer.MethodsImmunohistochemistry的组织，包括组织阵列，定量聚合酶链反应和mRNA表达数据库mining.ResultsIn小鼠前列腺上皮，C/EBPα是目前在出生后1周定位于胞浆，8周开始出现核定位，10周及以后继续显示显著的核表达; C/EBPα mRNA在所有年龄段均表达。在人类中，C/EBPα从青春期到中年表现出明显的核定位，但在老年个体中被隔离在细胞溶质中; C/EBPα的mRNA水平基本保持不变。大多数前列腺癌表达一系列的C/EBPα mRNA和蛋白质的水平是相对较高的转移性肿瘤的方式，与AR的表达;然而，大多数细胞显示C/EBPα在cytosol. ConclusionsTemporalchanges在亚细胞定位的C/EBP α是一致的前列腺分化的作用，并作为前列腺肿瘤抑制剂;老年人前列腺特有的C/EBPα的胞质隔离可以说是前列腺增生性疾病发生频率更高的一种允许条件。在恶性前列腺中，C/EBPα可能通过其亚细胞定位的瞬时变化来调节AR信号传导。前列腺68：1206-1214，2008。© 2008 Wiley利斯公司
BackgroundC/EBPα is a critical mediator of terminal differentiation and a tumor suppressor through its strong antiproliferative actions on cell cycle regulatory proteins. C/EBPα also appears to regulate androgen receptor (AR) AR signaling. There, is a paucity of information on the expression and sub‐cellular localization of C/EBPα in normal mouse and human prostate and in prostate cancer.MethodsImmunohistochemistry of tissues including tissue arrays, quantitative polymerase chain reaction and mRNA expression database mining.ResultsIn the mouse prostate epithelium, C/EBPα was present at 1 week postnatal localized in the cytosol, began to show nuclear localization at 8 weeks and continued to show prominent nuclear expression at 10 weeks and beyond; C/EBPα mRNA was expressed at all ages. In humans, C/EBPα showed prominent nuclear localization from peripubescence up to middle age but was sequestered in the cytosol in older individuals; the mRNA level for C/EBPα remained essentially unchanged. Most prostate adenocarcinomas expressed a range of levels of C/EBPα mRNA and protein that were relatively high in metastatic tumors in a manner that correlated with AR expression; however, most cells showed C/EBPα sequestered in the cytosol.ConclusionsTemporal changes in sub‐cellular localization of C/EBPα are consistent with a role in prostate differentiation and as a prostate tumor suppressor; the cytoplasmic sequestration of C/EBPα, unique to older human prostates, is arguably a permissive condition for the greater frequency of proliferative disorders of the prostate. In malignant prostate C/EBPα may be available to regulate AR signaling through transient changes in its sub‐cellular localization. Prostate 68: 1206–1214, 2008. © 2008 Wiley‐Liss, Inc.