Induction of oxidative stress by bisphenol A in the epididymal sperm of rats
Induction of oxidative stress by bisphenol A in the epididymal sperm of rats
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DOI：
10.1016/s0300-483x(02)00597-8
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发表时间：
2003-03-14
期刊：
影响因子：
4.5
通讯作者：
Mathur, PP
中科院分区：
文献类型：
--
作者：
Chitra, KC;Latchoumycandane, C;Mathur, PP
双酚 A 已被证明会影响雄性大鼠和小鼠的繁殖。然而，双酚A对附睾精子的作用机制尚未阐明。本研究旨在评估双酚A对大鼠附睾精子抗氧化系统的影响。雄性大鼠口服双酚A，剂量水平为每天0.2、2和20微克/公斤体重，持续45天。最后一次处理24小时后，对大鼠进行称重并使用麻醉乙醚处死。与相应的对照组相比，治疗组大鼠的体重没有显示出显着的变化。在双酚A治疗的大鼠中，睾丸和附睾的重量显着下降；与相应的对照组动物相比，腹侧前列腺的重量显着增加，而精囊的重量没有显着变化。从附睾收集的精子用于精子计数和生化评估。双酚A的施用导致附睾精子活力和精子数量以剂量依赖性方式减少。与相应对照组动物相比，治疗组大鼠超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶和谷胱甘肽过氧化物酶活性均显着降低，而H2O2和脂质过氧化水平显着升高。结果表明，分级剂量的双酚 A 会导致大鼠附睾精子抗氧化防御系统耗竭并诱发氧化应激。综上所述，双酚A对男性生殖的不利影响可能是由于诱导精子氧化应激所致。 (C) 2002 Elsevier Science Ireland Ltd. 保留所有权利。
Bisphenol A has been shown to affect the reproduction of male rats and mice. However, the mechanism of action of bisphenol A on the epididymal sperm is not elucidated. The present study was undertaken to evaluate the effect of bisphenol A on the antioxidant system of rat epididymal sperm. Bisphenol A was administered orally to male rats at the dose levels of 0.2, 2 and 20 mug/Kg body weight per day for 45 days. After 24 h of the last treatment, rats were weighed and killed using anesthetic ether. The body weight of treated rats did not show significant change as compared with the corresponding control groups. In bisphenol A-treated rats there was a significant decrease in the weight of the testis and epididymis; the weight of ventral prostate increased significantly whereas there was no significant change in the weight of seminal vesicles as compared with the corresponding group of control animals. Sperm collected from the epididymis were used for sperm count and biochemical estimations. Administration of bisphenol A caused a reduction in the epididymal sperm motility and sperm count in a dose-dependent manner. The activities of superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase were decreased while the levels of H2O2 and lipid peroxidation increased significantly in the treated rats as compared with the corresponding group of control animals. The results suggested that graded doses of bisphenol A elicit depletion of antioxidant defence system and induce oxidative stress in epididymal sperm of rats. In conclusion, the adverse effect of bisphenol A on male reproduction may be due to induction of oxidative stress in sperm. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.