Molecular heterogeneity in major urinary proteins of Mus musculus subspecies: potential candidates involved in speciation.
Molecular heterogeneity in major urinary proteins of Mus musculus subspecies: potential candidates involved in speciation.
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DOI：
10.1038/srep44992
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发表时间：
2017-03-24
影响因子：
4.6
通讯作者：
Ganem G
中科院分区：
文献类型：
--
作者：
Hurst JL;Beynon RJ;Armstrong SD;Davidson AJ;Roberts SA;Gómez-Baena G;Smadja CM;Ganem G
当杂交是有代价的，预计自然选择将有利于允许分类交配(强化)的特征的进化。家鼠和家鼠两个欧洲家鼠亚种之间的早期物种形成共享一个杂交区，为在分子水平上理解分类交配的进化提供了机会。老鼠尿液的气味允许亚种属配偶歧视，在杂交区有明显的分类偏好，但在异位反应中则不明显。在这里，我们通过比较来自丹麦杂交区边界(接触区)和来自异地人群的尿样中MUP的表达来评估MUP(主要尿蛋白)作为信号差异候选的可能性。质谱学鉴定在这两个亚种中发现了新的MUPs，主要涉及以前在家养分枝杆菌中观察到的氨基酸变化的新组合。亚种表现出不同的MUP特征，大多数MUP只由一个亚种表达。至少8个MUP的表达在异位和接触区均表现出明显的亚种分化。另外7个MUP仅在接触区表现出亚种间表达的差异，这与增强后的差异一致。这些蛋白质是杂交的信号化学屏障的候选者，为描述假定的物种识别信号的性质和进化提供了机会。
When hybridisation carries a cost, natural selection is predicted to favour evolution of traits that allow assortative mating (reinforcement). Incipient speciation between the two European house mouse subspecies, Mus musculus domesticus and M.m.musculus, sharing a hybrid zone, provides an opportunity to understand evolution of assortative mating at a molecular level. Mouse urine odours allow subspecific mate discrimination, with assortative preferences evident in the hybrid zone but not in allopatry. Here we assess the potential of MUPs (major urinary proteins) as candidates for signal divergence by comparing MUP expression in urine samples from the Danish hybrid zone border (contact) and from allopatric populations. Mass spectrometric characterisation identified novel MUPs in both subspecies involving mostly new combinations of amino acid changes previously observed in M.m.domesticus. The subspecies expressed distinct MUP signatures, with most MUPs expressed by only one subspecies. Expression of at least eight MUPs showed significant subspecies divergence both in allopatry and contact zone. Another seven MUPs showed divergence in expression between the subspecies only in the contact zone, consistent with divergence by reinforcement. These proteins are candidates for the semiochemical barrier to hybridisation, providing an opportunity to characterise the nature and evolution of a putative species recognition signal.