Herbivore‐induced Ca 2+ signals trigger a jasmonate burst by activating ERF16 ‐mediated expression in tomato
Herbivore‐induced Ca 2+ signals trigger a jasmonate burst by activating ERF16 ‐mediated expression in tomato
复制标题
草食动物诱导的 Ca 2 信号通过激活番茄中 ERF16 介导的表达来触发茉莉酸爆发
DOI：
10.1111/nph.18455
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发表时间：
2022
期刊：
影响因子：
9.4
通讯作者：
Jingquan Yu
中科院分区：
文献类型：
--
作者：
Chaoyi Hu;Shaofang Wu;Jiajia Li;Han Dong;Changan Zhu;Ting Sun;Zhangjian Hu;Christine H. Foyer;Jingquan Yu
食草病严重影响植物生长，对农作物生产构成威胁。在植物对食草动物攻击的反应中，钙离子(Ca~(2+))信号和茉莉酸(JAs)的积累被激活，导致防御途径的表达。本研究利用CRISPR/CAS9、UPLC-MS/MS和分子生物学等多种技术，研究了乙烯反应因子16在番茄草食防御过程中引发的JA猝发中的作用。在此，我们证明了模拟草食诱导依赖于GLUTAMATE受体LIKE3.3/3.5(GLR3.3/3.5)的GLUTAMATE受体LIKE3.3/3.5(GLR3.3/3.5)依赖的电活动增加，钙离子内流，并增加了番茄CALMODULIN2(CAM2)和ERF16转录本的丰度。CAM2和ERF16之间的相互作用通过增强ERF16的转录活性来促进JA的生物合成，ERF16的转录活性增加了ERF16的表达，并导致JA生物合成的关键基因Lipoxygennase D(LOXD)、AOC和12-OXO-植二酸还原酶3(OPR3)的表达。CaM2突变导致茉莉酸积累减少，与茉莉酸生物合成相关的基因表达一起，导致对棉铃虫抗性的降低。这些发现揭示了钙信号引发的系统性茉莉酸猝发的分子机制，并强调了钙信号/ERF16串扰在食草动物防御中的关键作用。
Herbivory severely affects plant growth, posing a threat to crop production. Calcium ion (Ca2+) signaling and accumulation of jasmonates (JAs) are activated in plant response to herbivore attack, leading to the expression of defense pathways. However, little is known about how the Ca2+signal modulates JA biosynthesis.We used diverse techniques, including CRISPR/Cas9, UPLC‐MS/MS and molecular biology methods to explore the role of ETHYLENE RESPONSE FACTOR 16 in Ca2+signal‐triggered JA burst during herbivore defense in tomato.Here we show that simulated herbivory inducesGLUTAMATE RECEPTOR LIKE3.3/3.5(GLR3.3/3.5)‐dependent increases in electrical activity, Ca2+influx and increases the abundance ofCALMODULIN2(CaM2) andERF16transcripts in tomato. The interaction between CaM2 and ERF16 promotes JA biosynthesis by enhancing the transcriptional activity of ERF16, which increases the activation ofERF16expression and causes expression ofLIPOXYGENASE D (LOXD),AOCand12‐OXO‐PHYTODIENOIC ACID REDUCTASE 3 (OPR3), the key genes in JA biosynthesis. Mutation ofCaM2results in decreased JA accumulation, together with the expression of JA biosynthesis‐related genes, leading to reduced resistance to the cotton bollwormHelicoverpa armigera.These findings reveal a molecular mechanism underpinning the Ca2+signal‐initiated systemic JA burst and emphasize the pivotal role of Ca2+signal/ERF16 crosstalk in herbivore defense.