Changes in vesicular glutamate transporter 2, vesicular GABA transporter and vesicular acetylcholine transporter labeling of sacrocaudal motoneurons in the spastic rat
Changes in vesicular glutamate transporter 2, vesicular GABA transporter and vesicular acetylcholine transporter labeling of sacrocaudal motoneurons in the spastic rat
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DOI：
10.1016/j.expneurol.2005.10.005
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发表时间：
2006-02-01
影响因子：
5.3
通讯作者：
Kitzman, P
中科院分区：
文献类型：
--
作者：
Kitzman, P
中线肌肉组织的痉挛可以显著地粘合执行转移并导致压疮的发展。目前，我们对脊髓损伤后痉挛发展的病理生理学的认识存在很大的差距，特别是关于轴向肌肉组织。本研究的目的是：（1）确定S-2横断对更多尾部运动神经元上的多巴胺能、GABA能和胆碱能输入的数量和分布的影响，（2）将这些变化与尾部肌肉系统（与轴肌系统对应的尾部肌肉系统）内痉挛的发展相关联。对S2脊髓横断动物的尾部肌肉组织内痉挛进展进行行为测试。在1，2，4，或12周损伤后，动物被处死，并使用抗体的特定囊泡转运蛋白的每种神经递质和共聚焦显微镜的骶尾侧运动神经元的GABA能，胆碱能和多巴胺能输入的时间变化进行了评估。损伤后1周，当尾部肌肉组织表现出反应性降低时，观察到骶尾运动神经元的兴奋性与抑制性输入的比例总体增加。从2至12周损伤后，当尾部肌肉表现出增加的反射行为时，观察到兴奋性与抑制性输入的比例总体下降。此外，脊髓横断后2 ~ 12周，观察到骶尾侧运动神经元胆碱能标记逐渐丧失。脊髓横断后兴奋的总体水平增加伴随着胆碱能影响的丧失，这可以部分解释尾部肌肉组织内痉挛的发展。(c)2005年爱思唯尔公司All rights reserved.
Spasticity of the midline musculature can significantly binder performing transfers and lead to development of pressure sores. Currently, significant gaps exist in Our knowledge of the pathophysiology involved in spasticity development following SCI, especially regarding the axial musculature. The goals of this study were: (1) to determine the effects of S-2 transection on the number and distribution of glutamatergic, GABAergic and cholinergic inputs on more caudal motoneurons, (2) to correlate these changes with the development of spasticity within the tail musculature, which are the caudal counterparts to the axial musculature. Animals with S2 spinal transection were tested behaviorally for the progression of spasticity within the tail musculature. At 1, 2, 4, or 12 weeks post-injury, the animals were sacrificed and temporal changes in glutamatergic, GABAergic, and cholinergic inputs to sacrocaudal motoneurons were assessed using antibodies for the specific vesicular transporter of each neurotransmitter and confocal microscopy. At I week post-injury, when the tail musculature demonstrated decreased responsiveness, an overall increase in the ratio of excitatory to inhibitory input to sacrocaudal motoneurons was observed. From 2 to 12 weeks post-injury when the tail Musculature demonstrated increased reflex behavior, an overall decrease in the ratio of excitatory to inhibitory inputs was observed. Additionally, from 2 to 12 weeks following spinal transection, a progressive loss of cholinergic labeling of sacrocaudal motoneurons was observed. The increase in the overall level of excitation with a concomitant loss of cholinergic influence following spinal transection could, in part, explain the development of spasticity within the tail musculature. (c) 2005 Elsevier Inc. All rights reserved.