Natural IgG antibody with anti-β-galactosyl specificity suppressed hepatoma cell invasion in culture.
Natural IgG antibody with anti-β-galactosyl specificity suppressed hepatoma cell invasion in culture.
复制标题
具有抗β-半乳糖基特异性的天然 IgG 抗体可抑制培养物中的肝癌细胞侵袭。
DOI：
10.1007/s10616-012-9523-5
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发表时间：
2012
期刊：
影响因子：
2.2
通讯作者：
Daisaburo Fujimoto
中科院分区：
文献类型：
--
作者：
Yutaka Miura;Hiroshi Fujita;Fumihiko Sakai;Hiroyuki Tachikawa;Kazumi Yagasaki;Daisaburo Fujimoto
研究了识别β-半乳糖表位的天然IgG抗体对肝癌细胞侵袭的影响。抗β-半乳糖抗体剂量依赖性地抑制肝癌在原代培养间皮细胞单层下的侵袭，而不影响细胞增殖，其抑制程度与具有抗α-半乳糖特异性的天然IgG抗体相同，已报道具有抗转移活性。在培养基中加入乳糖（半乳糖-β- 1,4 -葡萄糖）后，抗β-半乳糖抗体的抑制作用被完全抵消，说明该抗体识别了一些具有β-半乳糖表位的抗原。经该抗体预处理48 h的肝癌细胞侵袭活性降低，而经该抗体预处理的间皮细胞不影响肝癌细胞的侵袭。抗β-半乳糖抗体还能抑制肝癌细胞对间皮细胞单层的粘附。这些结果表明，具有抗β-半乳糖特异性的天然抗体可能识别肝癌细胞粘附相关分子中的β-半乳糖表位，从而抑制对间皮细胞单层的粘附和侵袭。这些结果提示这种抗体在转移性肿瘤的治疗中可能的治疗用途。
The effect of natural IgG antibody recognizing β-galactosyl epitope on hepatoma cell invasion was investigated. Anti-β-galactosyl antibody dose-dependently suppressed hepatoma invasion underneath primarily cultured mesothelial cells monolayer without affecting the proliferation, to the same extent as natural IgG antibody with anti-α-galactosyl specificity, which had already been reported to have an anti-metastatic activity. The inhibitory effect of anti-β-galactosyl antibody was completely canceled by adding lactose (galactose-β-1, 4-glucose) to the medium, indicating that this antibody recognized some antigens with β-galactosyl epitope. Hepatoma cells pretreated with this antibody for 48 h showed reduced invasive activity, while the pretreatment of mesothelial cells with the antibody did not affect hepatoma cells invasion. Anti-β-galactosyl antibody also suppressed hepatoma cells adhesion to mesothelial cells monolayer. These results suggest that natural antibody with anti-β-galactosyl specificity may recognize the β-galactosyl epitope in some adhesion-related molecules on hepatoma cells, thus suppressing adhesion and invasion to mesothelial cells monolayer. These results suggest possible therapeutic uses of this antibody in the treatment of metastatic tumors.