Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected at a Chinese hospital from 2011 to 2013.
Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected at a Chinese hospital from 2011 to 2013.
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2011-2013年中国某医院肺炎链球菌分离株血清型分布及耐药性分析
DOI：
10.1186/s12879-015-1042-5
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发表时间：
2015-08-05
影响因子：
3.7
通讯作者：
Yao Y
中科院分区：
文献类型：
--
作者：
Huang S;Liu X;Lao W;Zeng S;Liang H;Zhong R;Dai X;Wu X;Li H;Yao Y
背景：肺炎链球菌感染是全球发病率和死亡率的主要原因，耐药肺炎链球菌的出现越来越多地被报道。本研究提供了肺炎链球菌临床分离株细菌血清型分布及耐药性的最新信息，为我国肺炎链球菌病的防治策略提供指导。方法：收集2011-2013年中国某医院门诊和住院患者分离的94株肺炎链球菌。药物敏感性和耐药性由最低抑菌浓度(MICs)决定。结果：在94株肺炎链球菌临床分离株中，共鉴定出15种血清型。流行的血清型有19F(42.6%)、19A(8.5%)、3(8.5%)和6B(7.4%)。7价、10价和13价肺炎球菌多糖结合疫苗的潜在免疫接种率分别为59.6%、62.6%和79.6%。对四环素、红霉素和新诺明/磺胺甲恶唑的耐药率分别为91.2%、80.2%和63.8%。对青霉素、阿莫西林、头孢曲松和头孢噻肟的耐药率分别为47.3%、34.1%、19.8%和18.7%。在几乎所有病例中，5岁及以下患者分离的肺炎链球菌的耐药性高于51岁及以上患者分离的菌株。结论：94株肺炎链球菌临床分离株的流行血清型为19F、19A、3和6B型。13价肺炎球菌多糖结合疫苗覆盖了本样本中确定的大多数血清型。不同血清型和不同年龄段的耐药率不同。临床上应采取预防措施，以避免这种潜在的人类病原体产生多药耐药性。
Background:Streptococcus pneumoniae infections are a major cause of global morbidity and mortality, and the emergence of antibiotic-resistant Streptococcus pneumoniae strains has been increasingly reported. This study provides up-to-date information on bacterial serotype distribution and drug resistance from S. pneumoniae clinical isolates that could guide prevention and treatment strategies for pneumococcal disease in China.Methods:A total of 94 S. pneumoniae isolates were collected from outpatients and inpatients at one Chinese hospital from 2011-2013. Drug susceptibility and resistance was determined by minimum inhibitory concentrations (MICs). Capsular serotypes were identified by the quellung reaction test and multiplex polymerase chain reaction.Results:Fifteen serotypes were identified among the 94 S. pneumoniae clinical isolates that were collected. Prevalent serotypes were 19F (42.6 %), 19A (8.5 %), 3 (8.5 %), and 6B (7.4 %). Potential immunization coverage rates for the 7-, 10- and 13-valent pneumococcal polysaccharide conjugate vaccines were 59.6, 62.6, and 79.6 %, respectively. Resistance rates to tetracycline, erythromycin, and trimethoprim/sulfamethoxazole were 91.2, 80.2 and 63.8 %, respectively. Resistance rates to penicillin, amoxicillin, ceftriaxone, and cefotaxime were 47.3, 34.1, 19.8, and 18.7 %, respectively. In almost all cases, antimicrobial resistance of the S. pneumoniae isolates in patients five years or younger was higher than isolates collected from patients aged 51 years or older.Conclusion:Prevalent serotypes among the 94 S. pneumoniae clinical isolates were 19F, 19A, 3, and 6B. The 13-valent pneumococcal polysaccharide conjugate vaccine covered the majority of the serotypes identified in this sample. Drug resistance varied among different serotypes and age groups. Clinical precautions should be taken to avoid the development of multidrug resistance in this potential human pathogen.