Effects of transgenic cry1Ab rice pollen on fitness of Propylea japonica (Thunberg)
Effects of transgenic cry1Ab rice pollen on fitness of Propylea japonica (Thunberg)
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DOI：
10.1007/s10340-004-0078-x
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发表时间：
2005-06-01
影响因子：
4.8
通讯作者：
Cheng, JA
中科院分区：
文献类型：
--
作者：
Bai, YY;Jiang, MX;Cheng, JA
转Bt基因水稻克明稻1号（KMD 1）和克明稻2号（KMD 2）含有苏云金芽孢杆菌（Bacillus thuringiensis Berliner）的cry 1Ab基因，对水稻螟虫和食叶鳞翅目昆虫具有高度抗性。龟纹瓢虫（鞘翅目：瓢虫科（Coccinellidae）是水稻害虫的重要捕食者，在自然条件下也以水稻花粉为食物来源。在实验室条件下，研究了表达Cry 1Ab蛋白的KMD 1和KMD 2花粉对粳稻适合度的影响。以KMDI花粉和桃蚜、KMD 2花粉和桃蚜、KMD 3花粉和桃蚜、KMD 4花粉和桃蚜、KMD 2花粉和桃蚜、KMD 3花粉和桃蚜、KMD 2花粉和KMD 2花粉。将非转基因的秀水11号（KMD 1和KMD 2的亲本品种）花粉与M. persicae和M.桃蚜（分别为KMD 1-花粉、KMD 2-花粉、XS 11-花粉和蚜虫处理）。结果表明，KMD 1花粉处理的雌成虫寿命显著低于XS 11花粉处理，而与KMD 2花粉和蚜虫处理差异不显著。KMD 2花粉处理的新羽化雄虫的生命力明显低于XS 11花粉处理，但与KMD 1花粉和蚜虫处理的差异不显著。三种花粉处理的发育、存活和繁殖指数没有显著差异。总之，Bt水稻花粉中表达的Bt毒素对该虫的适合度没有明显的负面影响。
The transgenic rice lines Kemingdao 1 (KMD 1) and Kemingdao 2 (KMD2) contain a synthetic cry1Ab gene derived from Bacillus thuringiensis Berliner and are highly resistant to rice stem borers and foliage-feeding lepidopterans. Propylea japonica (Coleoptera: Coccinellidae) is an important predator of rice insect pests; it also uses rice pollen as a food source under natural conditions. In the present study, the effects of KMD1 and KMD2 pollen expressing Cry1Ab protein on the fitness of P. Japonica were assessed in the laboratory. P. Japonica larvae and adults were provided with the following four diets: KMDI pollen with the aphid Myzus persicae, KMD2 pollen with M. persicae, nontransgenic Xiushui 11 (parent variety of KMD1 and KMD2) pollen with M. persicae, and M. persicae only (KMD1-pollen, KMD2-pollen, XS11-pollen, and aphid treatments, respectively). The results showed that the longevity of female adults in the KMD1-pollen treatment was significantly lower than that in the XS11-pollen treatment, but was not significantly different from that in the KMD2-pollen and aphid treatments. Newly emerged males in the KMD2-pollen treatment were evidently less vital than those in the XS11-pollen treatment, but not significantly different from those in the KMD1-pollen and aphid treatments. The development, survival and reproduction indices for the three pollen treatments did not differ significantly from one another. In short, Bt toxin expressed in Bt rice pollen had no evident negative impacts on P. japonica fitness when the pollen was used as a food by this beetle.