Bradykinin-induced changes in myo-inositol 1,2-(cyclic)phosphate in rabbit papillary collecting tubule cells.
Bradykinin-induced changes in myo-inositol 1,2-(cyclic)phosphate in rabbit papillary collecting tubule cells.
复制标题
缓激肽诱导兔乳头集合管细胞中肌醇 1,2-（环）磷酸的变化。
DOI：
10.1016/0167-4889(86)90018-2
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Morrison,AR
中科院分区：
文献类型：
--
作者：
Shayman,JA;Auchus,RJ;Morrison,AR
收获兔肾乳头状集合管细胞并在原代培养物中生长。当用肌-[2- 3 H]肌醇标记并在中性条件下提取时，通过阴离子交换色谱分离观察到在酸性提取下未检出的代谢产物，并在类似条件下用真实的1-肌-肌醇1，2-（环）磷酸盐洗脱;其五（三甲基甲硅烷基）衍生物的质谱包含与真实标准品相同比例的选定离子片段。缓激肽，以前证明增加标记的游离肌醇多磷酸，增加标记的肌醇1，2环磷酸，但在一个时间过程后，形成肌醇三磷酸。这些观察结果与缓激肽诱导磷脂酰肌醇4，5-二磷酸水解的模型一致，该模型先于肾乳头状集合小管细胞中磷脂酰肌醇的水解。
Rabbit renal pipillary collecting tubule cells were harvested and grown in primary cultures. When labeled withmyo-[2-3H]inositol and extracted under neutral conditions, a metabolite undetected under acidic extraction was observed on resolution by anion-exchange chromatography and which eluted under similar conditions with authenticdl-myo-inositol 1,2-(cyclic)phosphate; the mass spectrum of its pentakis(trimethylsilyl) derivative contains an identical ratio of selected ion fragments to the authentic standard. Bradykinin, demonstrated previously to increase labeling of free inositol polyphosphates, increases labeling of inositol 1,2 cyclic phosphate but over a time course subsequent to the formation of inositol trisphosphate. These observations are consistent with the model that bradykinin induces hydrolysis of phosphatidylinositol 4,5-bisphosphate which precedes hydrolysis of phosphatidylinositol in renal papillary collecting tubule cells.