Proteomics Fingerprinting of Phagosome Maturation and Evidence for the Role of a Gα during Uptake*S
Proteomics Fingerprinting of Phagosome Maturation and Evidence for the Role of a Gα during Uptake*S
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吞噬体成熟的蛋白质组学指纹图谱和 Gα 在摄取过程中作用的证据*S
DOI：
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发表时间：
2006
影响因子：
7
通讯作者：
T. Soldati
中科院分区：
文献类型：
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作者：
D. Gotthardt;V. Blancheteau;A. Bosserhoff;T. Ruppert;M. Delorenzi;T. Soldati
吞噬作用，无论是原生动物中的食物颗粒还是后生动物免疫系统中的细菌和细胞残余物，都是一个保守的过程。这些颗粒被吞噬体吸收，然后吞噬体对其成分进行复杂的重塑，称为成熟。通过二维凝胶电泳和质谱结合基因组数据，我们鉴定了阿米巴盘基网柄菌中的 179 个吞噬体蛋白，包括信号转导、膜交通和细胞骨架的成分。通过在成熟过程中进行蛋白质组学分析，我们获得了 1,388 个点的时间曲线，从而生成了吞噬体蛋白质组成的动态记录。时间概况的聚类揭示了 5 个聚类和 24 个功能组，它们被映射到成熟流程图上。最早出现的两个异源三聚体G蛋白亚基Gα4和Gβ。我们发现，编码这两种蛋白质的基因突变会导致盘基网柄菌的吞噬细胞摄取缺陷。对吞噬体蛋白动力学的分析为未来的遗传和功能研究提供了参考点。
Phagocytosis, whether of food particles in protozoa or bacteria and cell remnants in the metazoan immune system, is a conserved process. The particles are taken up into phagosomes, which then undergo complex remodeling of their components, called maturation. By using two-dimensional gel electrophoresis and mass spectrometry combined with genomic data, we identified 179 phagosomal proteins in the amoeba Dictyostelium, including components of signal transduction, membrane traffic, and the cytoskeleton. By carrying out this proteomics analysis over the course of maturation, we obtained time profiles for 1,388 spots and thus generated a dynamic record of phagosomal protein composition. Clustering of the time profiles revealed five clusters and 24 functional groups that were mapped onto a flow chart of maturation. Two heterotrimeric G protein subunits, Gα4 and Gβ, appeared at the earliest times. We showed that mutations in the genes encoding these two proteins produce a phagocytic uptake defect in Dictyostelium. This analysis of phagosome protein dynamics provides a reference point for future genetic and functional investigations.
Rab11 样 GTPase 与网柄菌收缩液泡系统的结构和功能相关并调节其结构和功能。
影响因子：
4
作者：
Harris,E;Yoshida,K;Cardelli,J;Bush,J
通讯作者：
Bush,J