Papillote and Piopio:: Drosophila ZP-domain proteins required for cell adhesion to the apical extracellular matrix and microtubule organization
Papillote and Piopio:: Drosophila ZP-domain proteins required for cell adhesion to the apical extracellular matrix and microtubule organization
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DOI：
10.1242/jcs.01619
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发表时间：
2005-02-01
影响因子：
4
通讯作者：
Brown, NH
中科院分区：
文献类型：
--
作者：
Bökel, C;Prokop, A;Brown, NH
上皮细胞和细胞外基质之间的粘附通常通过基底粘附复合物介导。然而，一些细胞在其顶端表面上也具有类似的连接。在这里，我们描述了两个新的果蝇蛋白质，Piopio和Papillote，这是所需的顶端上皮表面和覆盖顶端细胞外基质（aECM）之间的联系。这两种蛋白质与哺乳动物aECM组分（包括在脊椎动物内耳中发现的tectorins）共享透明质酸（ZP）结构域。两种蛋白质的标记版本定位于顶端上皮表面。piopio、papillote和dumpy（另一种编码ZP结构域蛋白的基因）中的突变导致aECM最内层的缺陷及其从表皮的脱离。失去Piopio，但不是Papillote或Dumpy，导致专门的微管束从蛹翅的情况下，这表明Piopio在微管组织中发挥作用。因此，含有ZP结构域的蛋白质可能在aECM内具有共享功能，同时还表现出与细胞骨架的特异性相互作用。
Adhesion between epithelial cells and extracellular substrates is normally mediated through basal adhesion complexes. However, some cells also possess comparable junctions on their apical surface. Here, we describe two new Drosophila proteins, Piopio and Papillote, that are required for the link between the apical epithelial surface and the overlying apical extracellular matrix (aECM). The two proteins share a zona pellucida (ZP) domain with mammalian aECM components, including the tectorins found in the vertebrate inner ear. Tagged versions of both proteins localized to the apical epithelial surface. Mutations in piopio,papillote and dumpy (another gene encoding a ZP-domain protein) cause defects in the innermost layer of the aECM and its detachment from the epidermis. Loss of Piopio, but not Papillote or Dumpy, causes the absence of specialized microtubule bundles from pupal wings, suggesting that Piopio plays a role in microtubule organization. Thus, ZP domain-containing proteins may have shared functions within the aECM, while also exhibiting specific interactions with the cytoskeleton.