STUDIES ON DEVELOPMENT OF MOUSE EMBRYOS IN VITRO .I. EFFECT OF OSMOLARITY AND HYDROGEN ION CONCENTRATION
STUDIES ON DEVELOPMENT OF MOUSE EMBRYOS IN VITRO .I. EFFECT OF OSMOLARITY AND HYDROGEN ION CONCENTRATION
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DOI：
10.1002/jez.1401580105
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发表时间：
1965-01-01
影响因子：
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通讯作者：
BRINSTER, RL
中科院分区：
文献类型：
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作者：
BRINSTER, RL
对几千个小鼠2-细胞卵进行了体外培养，观察了改变培养液的渗透压和氢离子浓度对发育的影响。在整个实验中采用统计实验设计技术，以最有效地利用现有的材料。将2细胞卵培养3天，并将发育成正常胚泡的百分比用作治疗效果的量度。改变培养液的渗透压对小鼠2-细胞卵发育成囊胚的数量有显著影响。囊胚在渗透压范围为0.2002和0.3542渗透压的培养基中发育。小鼠2-细胞卵发育为囊胚的最适渗透压为0.2760 osmol。培养基的氢离子浓度也对2细胞小鼠卵子发育为囊胚有影响。虽然囊胚在pH值为5.87 - 7.78的培养基中发育，但最适pH值似乎接近6.82。
Several thousand 2-cell mouse ova were cultivated in vitro, and the effect on development on changing the osmolarity and hydrogen ion concentration of the medium was examined. Statistical experimental design techniques were employed throughout the experiments in order to make the most efficient use of the material available. The 2-cell ova were cultivated for 3 days, and the percentage which developed into normal blastocysts was used as a measure of the treatment effect. Changing the osmolarity of the medium had a marked effect on the number of blastocysts developing from 2-cell mouse ova. Blastocysts developed in media with osmolarities ranging between 0.2002 and 0.3542 osmols. The optimum osmolarity for the development of 2-cell mouse ova into blastocysts was 0.2760 osmols. The hydrogen ion concentration of the culture medium also had an effect on the development of 2-cell mouse ova into blastocysts. Although blastocysts developed in media with pH''s ranging from 5.87 to 7.78, the optimum pH appeared to be near 6.82.