Functional characterization of Ostreococcus tauri phototropin.
Functional characterization of Ostreococcus tauri phototropin.
复制标题
金牛座向光素的功能表征。
DOI：
10.1111/nph.13640
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Sullivan S
中科院分区：
文献类型：
--
作者：
Sullivan S
向光素（phototropin）调节植物的一系列适应过程，从而优化光合作用效率并促进生长。拟南芥的光感应主要由位于光感受器 N 末端的光、氧和电压感应 2 (LOV2) 黄素结合基序介导。在这里，我们表征了来自海洋超微藻Ostreococcus tauriphototropin (Otphot) 的光的光化学和生化特性，并检查了其替代拟南芥中光介导功能的能力。Otphot 的光化学特性依赖于 LOV1 和 LOV2。然而，生化分析表明光依赖性受体自身磷酸化主要依赖于 LOV2。正如在拟南芥中发现的那样，Otphot 与质膜结合，并在蓝光照射下部分内化，尽管程度有限。Otphot 能够在拟南芥中引发许多光调节过程，包括叶柄定位、叶片扩张、气孔打开和叶绿体积累运动。然而，Otphot 无法恢复向光性和叶绿体回避运动。与拟南芥中缺乏向光功能一致，Otphot 不会与向光信号成分非向光性下胚轴 3 (NPH3) 相关或触发去磷酸化。总而言之，这些发现表明植物和进化上遥远的藻类光的作用机制并不像以前认为的那样保守。
Phototropins (phots) regulate a range of adaptive processes in plants that serve to optimize photosynthetic efficiency and promote growth. Light sensing byArabidopsis thalianaphots is predominantly mediated by the Light, Oxygen and Voltage sensing 2 (LOV2) flavin‐binding motif located within the N‐terminus of the photoreceptor. Here we characterize the photochemical and biochemical properties of phot from the marine picoalgaOstreococcus tauriphototropin (Otphot) and examine its ability to replace phot‐mediated function inArabidopsis.Photochemical properties of Otphot rely on both LOV1 and LOV2. Yet, biochemical analysis indicates that light‐dependent receptor autophosphorylation is primarily dependent on LOV2. As found forArabidopsisphots, Otphot associates with the plasma membrane and partially internalizes, albeit to a limited extent, in response to blue‐light irradiation.Otphot is able to elicit a number of phot‐regulated processes inArabidopsis, including petiole positioning, leaf expansion, stomatal opening and chloroplast accumulation movement. However, Otphot is unable to restore phototropism and chloroplast avoidance movement. Consistent with its lack of phototropic function inArabidopsis, Otphot does not associate with or trigger dephosphorylation of the phototropic signalling component Non‐Phototropic Hypocotyl 3 (NPH3).Taken together, these findings indicate that the mechanism of action of plant and evolutionarily distant algal phots is less well conserved than previously thought.
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影响因子：
3.4
作者：
Guo, HM;Kottke, T;Dick, B
通讯作者：
Dick, B
来自海藻金牛骨球菌的新型趋光素的 LOV1 结构域中的保守异亮氨酸可调节该结构域的暗态恢复。
DOI：
10.1016/j.bbagen.2011.04.008
发表时间：
2011
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
S. K. Veetil;Chitvan Mittal;Peeyush Ranjan;S. Kateriya
通讯作者：
S. Kateriya
影响因子：
4.8
作者：
Jones, Matthew A.;Feeney, Kevin A.;Christie, John M.
通讯作者：
Christie, John M.
影响因子：
11.6
作者：
LISCUM, E;BRIGGS, WR
通讯作者：
BRIGGS, WR
揭示 NPH3/RPT2 样蛋白在向光素信号转导中的功能1[CC-BY]
影响因子：
7.4
作者：
J. Christie;Noriyuki Suetsugu;S. Sullivan;M. Wada
通讯作者：
M. Wada