Influence of stressors on normal intestinal microbiota, intestinal morphology, and susceptibility to Salmonella Enteritidis colonization in broilers
Influence of stressors on normal intestinal microbiota, intestinal morphology, and susceptibility to Salmonella Enteritidis colonization in broilers
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DOI：
10.3382/ps.2008-00107
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发表时间：
2008-09-01
期刊：
影响因子：
4.4
通讯作者：
Patterson, J. A.
中科院分区：
文献类型：
--
作者：
Burkholder, K. M.;Thompson, K. L.;Patterson, J. A.
在现代家禽生产系统中，环境应激因素可能会影响家禽的生产性能和对沙门氏菌等病原体的易感性。本试验研究了24 h停饲和24 h高温暴露对肉仔鸡肠道特性的影响。使用体外回肠环测定法测定肠道沙门氏菌与回肠组织的附着。采用变性梯度凝胶电泳测定回肠微生物种群的变化，并通过组织学方法测定回肠形态的改变。在禁食24小时的肉鸡中，肠沙门氏菌与回肠组织的离体附着增加了1.5 log（9.05 log（10）vs. 7.59 log（10）沙门氏菌/g回肠组织; P = 0.0006）。类似地，与保持在23 ℃下的鸟相比，来自经历30 ℃下24小时的鸟的回肠组织具有增加的肠道沙门氏菌的离体附着（8.77 log（10）对8.50 log（10）肠道沙门氏菌/g回肠; P = 0.01）。暴露于30 ℃ 24小时也改变了回肠和盲肠中的微生物群落结构。将鸟类在30 ℃下24小时，降低了隐窝深度（分别为6.0和7.8 μ m; P = 0.002），但对绒毛高度或绒毛：隐窝比没有影响。这项研究表明，家禽生产系统中的急性应激源会导致正常肠道微生物群和上皮结构的变化，这可能导致沙门氏菌的附着增加。
In modern poultry production systems, environmental stressors may influence bird performance and susceptibility to pathogens such as Salmonella Enteritidis. Experiments were conducted to determine the influence of 24-h feed withdrawal and 24-h exposure to high temperature (30 degrees C) on intestinal characteristics of broilers. Attachment of Salmonella Enteritidis to ileal tissue was determined using an in vitro ileal loop assay. Changes in commensal intestinal microbial populations were determined using denaturing gradient gel electrophoresis, and alterations in ileal morphology were determined histologically. Ex vivo attachment of Salmonella Enteritidis to ileal tissues increased by 1.5 logs (9.05 log(10) vs. 7.59 log(10) Salmonella Enteritidis/g of ileal tissue; P = 0.0006) in broilers fasted for 24 h. Similarly, ileal tissues from birds subjected to 30 degrees C for 24 h had increased ex vivo attachment of Salmonella Enteritidis (8.77 log(10) vs. 8.50 log(10) Salmonella Enteritidis/g of ileum; P = 0.01) compared with birds held at 23 degrees C. Exposure to 30 degrees C for 24 h also altered microbial community structure in the ileum and cecum. Subjecting birds to 30 degrees C for 24 h reduced crypt depth (6.0 vs. 7.8 mu m, respectively; P = 0.002), but had no effect on villus height or villus: crypt ratio. This research shows that acute stressors in poultry production systems can cause changes in the normal intestinal microbiota and epithelial structure, which may lead to increased attachment of Salmonella Enteritidis.