RAT MIDDLE CEREBRAL-ARTERY OCCLUSION - EVALUATION OF THE MODEL AND DEVELOPMENT OF A NEUROLOGIC EXAMINATION
RAT MIDDLE CEREBRAL-ARTERY OCCLUSION - EVALUATION OF THE MODEL AND DEVELOPMENT OF A NEUROLOGIC EXAMINATION
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DOI：
10.1161/01.str.17.3.472
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发表时间：
1986-05-01
期刊：
影响因子：
8.3
通讯作者：
BARTKOWSKI, H
中科院分区：
文献类型：
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作者：
BEDERSON, JB;PITTS, LH;BARTKOWSKI, H
我们已经研究了梗塞的发病率和大小后，闭塞的不同部分的大鼠大脑中动脉（MCA），以确定该模型的可靠性和可预测性的脑缺血。我们进行了神经学检查，并将神经功能状态的变化与梗死面积的大小和位置相关联。在六组正常大鼠的不同部位手术闭塞MCA。24小时后，评价大鼠的神经功能缺损程度，并使用针对该模型开发的新检查将其分级为具有重度、中度或无缺损。处死大鼠后，通过组织学检查确定梗死发生率。在一组大鼠中，梗死面积的大小被测量为标准冠状切片面积的百分比。大脑中动脉起源处、嗅束处或大脑下静脉外侧的局灶性（1-2 mm）闭塞分别在13%、67%和0%的大鼠中产生梗死（N = 38），并产生不同的神经功能缺损。然而，更广泛（3或6毫米）闭塞MCA开始近端嗅束，从而隔离的近端和远端供应的纹状体终末动脉，产生梗死的大小，位置均匀，并在100%的大鼠（N = 17）严重的神经功能缺损（2级）。神经功能缺损与梗死面积显著相关（2级，N = 17，28 . ±. 5%梗死; 1级，N = 5，19 . ±. 5%; 0级，N = 3，10 . ±. 05）。我们的特点是精确的解剖部位的大脑中动脉，当手术闭塞可靠地产生均匀的脑梗死大鼠，并已开发出一种神经系统分级系统，可用于评估脑缺血的影响迅速和准确。该模型将有助于不可逆性脑缺血新疗法的实验评估。
We have examined the incidence and size of infarction after occlusion of different portions of the rat middle cerebral artery (MCA) in order to define the reliability and predictability of this model of brain ischemia. We developed a neurologic examination and have correlated changes in neurolgic status with the size and location of areas of infarction. The MCA was surgically occluded at different sites in six groups of normal rats. After 24 hr, rats were evaluated for the extent of neurologic deficits and graded as having severe, moderate, or no deficit using a new examination developed for this model. After rats were sacrificed the incidence of infarction was determined at histologic examination. In a subset of rats, the size of the area of infarction was measured as a percent of the area of a standard coronal section. Focal (1-2 mm) occlusion of the MCA at its origin, at the olfactory tract, or lateral to the inferior cerebral vein produced infarction in 13%, 67%, and 0% of rats, respectively (N = 38) and produced variable neurologic deficits. However, more extensive (3 or 6 mm) occlusion of the MCA beginning proximal to the olfactory tract-thus isolating lenticulostriate end-arteries from the proximal and distal supply-produced infarctions of uniform size,location, and with severe neurologic deficit (Grade 2) in 100% of rats (N = 17). Neurologic deficit correlated significantly with the size of the infracted area (Grade 2, N = 17, 28 .+-. 5% infarction; Grade 1, N = 5, 19 .+-. 5%; Grade 0, N = 3, 10 .+-. 2%; p < 0.05). We have characterized precise anatomical sites of the MCA that when surgically occluded reliably produce uniform cerebral infarction in rats, and have developed a neurologic grading system that can be used to evaluate the effects of cerebral ischemia rapidly and accurately. The model will be useful for experimental assessment of new therapies for irreversible cerebral ischemia.