Dansylation of amino acids for high-performance liquid chromatography analysis.
Dansylation of amino acids for high-performance liquid chromatography analysis.
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DOI：
10.1016/0003-2697(81)90534-0
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发表时间：
1981-07
影响因子：
2.9
通讯作者：
Y. Tapuhi;Donald E. Schmidt;Wolfgang Lindner;Barry L. Karger
中科院分区：
文献类型：
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作者：
Y. Tapuhi;Donald E. Schmidt;Wolfgang Lindner;Barry L. Karger
提出了一种与反相高效液相色谱兼容的氨基酸丹磺酰化方法。该方法提供了高产率的衍生化，而在1000倍的范围内，产率似乎是独立的丹磺酰氯与氨基酸的比例。研究衍生化产率作为反应时间的函数在环境温度下，并在升高的温度下允许适当的选择反应条件。丹磺酰-液相色谱法、气相色谱法和氨基酸分析仪分析复杂细菌肉汤中氨基酸的方法的比较研究证明了丹磺酰-液相色谱法的有利使用。
An amino acid dansylation procedure compatible with reversed-phase high-performance liquid chromatography is presented. The method provides high yield derivatization while the yield appears to be independent of the ratio of dansyl chloride to amino acid over a 1000-fold range. Studies of derivatization yields as a function of reaction time at ambient temperature and at elevated temperatures permit the proper choice of reaction conditions. A comparative study of the dansyl-liquid chromatography, gas chromatography, and amino acid analyzer methods of analysis for amino acids in complex bacteria broths demonstrates the advantageous use of the dansyl-liquid chromatography approach.