Enhanced Inhibitory Effects of TBT Chloride on the Development of F1 Rats
Enhanced Inhibitory Effects of TBT Chloride on the Development of F1 Rats
复制标题
DOI：
10.1007/s00244-009-9421-9
复制
发表时间：
2010-05-01
影响因子：
4
通讯作者：
Aizawa, Y.
中科院分区：
文献类型：
--
作者：
Asakawa, H.;Tsunoda, M.;Aizawa, Y.
神经毒性是三丁基锡的主要效应之一。通过胎盘和母鼠乳汁接触三丁基锡化合物的F-1代大鼠的下一代受到的影响可能比成年大鼠更大。怀孕的Wister大鼠在其食物中接触到百万分之0和125的三丁基锡化合物。两组中有一半的雌性F-1大鼠在9至15周龄时在食物中接触到百万分之125的三丁基锡化合物。将F-1雌性大鼠分为以下几组：对照-对照组（CC），不接触三丁基锡化合物;三丁基锡化合物-对照组（TC），通过胎盘和母鼠乳汁接触三丁基锡化合物;对照-三丁基锡化合物（CT）组，从9至15周龄通过食物接触三丁基锡化合物; TBT-TBT（TT）组，通过胎盘、母奶和食物暴露于TBT（n = 10/组）。给药后，在15周龄时进行旷场试验和前脉冲抑制（PPI）试验。从9 ~ 15周龄，TC组和TT组的平均体重显著低于CC组。TC和TT组的平均相对胸腺重量显著低于CC组。在旷场试验中，观察到TT组的总运动距离显著减少。TT和TC组的平均值显著低于CC组。对于15 - 20 min的运动距离，CT、TC和TT组的平均值显著低于CC组。TT组25 ~ 30 min的平均运动距离显著低于CC组和TC组。TC、CT和TT组的室壁后仰次数平均值显著低于CC组。TT组的洗脸或洗澡次数均值显著低于CT组。PPI试验各项指标无显著性差异。通过胎盘和母鼠的乳汁接触三丁基锡化合物会抑制F-1代大鼠的发育，这种情况在断奶后继续存在。研究表明，通过胎盘和母鼠乳汁和/或通过食物接触三丁基锡化合物会抑制大鼠的活动。TT组的效果最明显。
Neurotoxicity is one of the major effects of tributyltin (TBT). The effects on the next generation of F-1 rats exposed to TBT via the placenta and their dams' milk may be stronger than those on adults. Pregnant Wister rats were exposed to TBT at 0 and 125 ppm in their food. Half of the female F-1 rats in both groups were exposed to TBT at 125 ppm in their food from 9 to 15 weeks of age. Female F-1 rats were divided into the following groups: the control-control (CC) group, with no exposure; the TBT-control (TC) group, exposed to TBT via the placenta and their dams' milk; the control-TBT (CT) group, exposed to TBT via their food from 9 to 15 weeks of age; and the TBT-TBT (TT) group, exposed to TBT via the placenta, their dams' milk, and their food (n = 10/group). After administration, an open-field test and prepulse inhibition (PPI) test were performed at 15 weeks of age. The mean body weights of the TC and TT groups were significantly lower than that of the CC group from 9 to 15 weeks of age. The mean relative thymus weight of the TC and TT groups was significantly lower than that of the CC group. In the open-field test, a marked decrease in the total locomotion distance was observed in the TT group. The mean values in the TT and TC groups were significantly lower than that in the CC group. For the locomotion distance between 15 and 20 min, the mean values in the CT, TC, and TT groups were significantly lower than that in the CC group. The mean locomotor distance between 25 and 30 min in the TT group was significantly lower than that in the CC and TC groups. The mean values of instances of wall rearing in the TC, CT, and TT groups were significantly lower than that in the CC group. The mean value of face washing or body washing in the TT group was significantly lower than that in the CT group. There were no significant differences in indexes of the PPI test. Exposure to TBT via the placenta and their dams' milk inhibited the development of F-1 rats, which continued after weaning. Inhibition of the rats' activity induced by exposure to TBT via the placenta and their dams' milk and/or via their food was suggested. The effects were most evident in the TT group.