Distribution and characterization of calcitonin gene-related peptide immunoreactivity in the digestive system of normal and capsaicin-treated rats.
Distribution and characterization of calcitonin gene-related peptide immunoreactivity in the digestive system of normal and capsaicin-treated rats.
复制标题
正常大鼠和辣椒素治疗大鼠消化系统中降钙素基因相关肽免疫反应性的分布和特征。
DOI：
10.1016/0016-5085(87)90450-1
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发表时间：
1987
期刊：
影响因子：
29.4
通讯作者：
Brecha,N
中科院分区：
文献类型：
--
作者：
Sternini,C;ReeveJr,JR;Brecha,N
采用放射免疫分析、层析和免疫组织化学方法，研究了正常大鼠、辣椒素处理大鼠和同窝对照大鼠消化系统降钙素基因相关肽免疫反应性的分布和特征。降钙素基因相关肽免疫反应性的最高浓度见于胃（45 ± 2.8 pmol/g湿重，非分泌区; 38.7 ± 4.4 pmol/g湿重，分泌区）和直肠（30.9 ± 1.6 pmol/g湿重）。在肠道的其他区域和胰腺中也发现了大量的肽。与同窝对照组相比，用辣椒素治疗新生儿，导致大多数小直径感觉神经元的永久变性，使食管和胃中的降钙素基因相关肽含量减少> 95%，胰腺中减少60%，肠中减少< 50%。通过色谱法分离肠、胰腺和脑的提取物，得到与预测的大鼠降钙素基因相关肽1 - 37相对应的主峰和推测由肽代谢产生的未识别的小峰。免疫组织化学研究表明，在食管和胃，降钙素基因相关肽的免疫反应性仅限于神经纤维，而在肠道中，它是本地化的神经纤维和肠神经节细胞。在辣椒素处理的大鼠中，几乎完全消除了支配食管和胃的降钙素基因相关肽免疫反应性纤维，而在小肠和大肠中，与血管相关的免疫反应性纤维显著减少，并且通常完全消除，非血管免疫反应性纤维略有减少。本研究的结果表明，降钙素基因相关肽免疫反应神经纤维支配大鼠消化系统起源于内在（肠）和外在（推测感觉）的来源，这两个内在和外在的组件似乎含有一种物质，对应于预测的降钙素基因相关肽1 -37。
The distribution and characterization of calcitonin gene-related peptide immunoreactivity in the digestive system of normal, capsaicin-treated, and littermate control rats were studied by radioimmunoassay, chromatography, and immunohistochemistry. The highest concentrations of calcitonin generelated peptide immunoreactivity were found in the stomach (45 ± 2.8 pmol/g wet wt, nonsecretory region; 38.7 ± 4.4 pmol/g wet wt, secretory region) and rectum (30.9 ± 1.6 pmol/g wet wt). Significant amounts of peptide were also found in the other regions of the gut and in the pancreas. Neonatal treatment with capsaicin, which causes a permanent degeneration of most of the small-diameter sensory neurons, reduced calcitonin gene-related peptide content by > 95% in the esophagus and stomach, by 60% in the pancreas, and by < 50% in the intestine, when compared with littermate controls. Separation of extracts from the gut, pancreas, and brain by chromatography gave major peaks corresponding to the predicted rat calcitonin gene-related peptide1–37and small unidentified peaks, which presumably arise from metabolism of the peptide. Immunohistochemical studies demonstrated that in the esophagus and stomach, calcitonin gene-related peptide immunoreactivity is restricted to nerve fibers, whereas in the intestine it is localized in both nerve fibers and enteric ganglion cells. In capsaicintreated rats there was a virtually complete elimination of calcitonin gene-related peptide immunoreactive fibers innervating the esophagus and stomach, whereas in the small and large intestine there was a dramatic reduction and often a complete elimination of those associated with blood vessels and a slighter reduction of the nonvascular immunoreactive fibers. The results of this study indicate that calcitonin gene-related peptide immunoreactive nerve fibers innervating the rat digestive system originate from both intrinsic (enteric) and extrinsic (presumably sensory) sources and that both the intrinsic and extrinsic components appear to contain a substance that corresponds to the predicted calcitonin gene-related peptide1–37.