Bph6 encodes an exocyst- localized protein and confers broad resistance to planthoppers in rice
Bph6 encodes an exocyst- localized protein and confers broad resistance to planthoppers in rice
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Bph6 编码一种外囊定位蛋白，赋予水稻对飞虱的广泛抗性
DOI：
10.1038/s41588-018-0039-6
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发表时间：
2018-02-01
期刊：
影响因子：
30.8
通讯作者：
He, Guangcun
中科院分区：
文献类型：
--
作者：
Guo, Jianping;Xu, Chunxue;He, Guangcun
褐飞虱(BPH)和白背飞虱(WBPH)是水稻上最具破坏性的害虫，对整个亚洲的水稻生产构成严重威胁。因此，迫切需要鉴定抗性相关基因，培育抗稻飞虱品种。在这里，我们报道了基于图谱的Bph6基因的克隆和功能分析，Bph6基因是水稻对稻飞虱的抗性基因。Bph6编码一种以前未知的蛋白质，定位于胞外包囊，并与胞外包囊亚单位OsEXO70E1相互作用。Bph6的表达增加胞吐作用，并参与细胞壁的维持和加固。在携带Bph6的植物中激活了协调的细胞分裂素、水杨酸和茉莉酸信号通路，这些植物在不牺牲产量的情况下对所有测试的褐飞虱生物型和白褐飞虱表现出广泛的抗性，因为这些植物被发现在褐飞虱严重侵染的领域保持着高水平的表现。我们的结果表明，在一年四季都能发现稻飞虱的地区，很久以前进化出的一种优越的抗性基因，可能对控制农业害虫非常有价值。
The brown planthopper (BPH) and white-backed planthopper (WBPH) are the most destructive insect pests of rice, and they pose serious threats to rice production throughout Asia. Thus, there are urgent needs to identify resistance-conferring genes and to breed planthopper-resistant rice varieties. Here we report the map-based cloning and functional analysis of Bph6, a gene that confers resistance to planthoppers in rice. Bph6 encodes a previously uncharacterized protein that localizes to exocysts and interacts with the exocyst subunit OsEXO70E1. Bph6 expression increases exocytosis and participates in cell wall maintenance and reinforcement. A coordinated cytokinin, salicylic acid and jasmonic acid signaling pathway is activated in Bph6-carrying plants, which display broad resistance to all tested BPH biotypes and to WBPH without sacrificing yield, as these plants were found to maintain a high level of performance in a field that was heavily infested with BPH. Our results suggest that a superior resistance gene that evolved long ago in a region where planthoppers are found year round could be very valuable for controlling agricultural insect pests.