The effects of alcohols, procaine and hyperthermia on the protein content of nuclei and chromatin.
The effects of alcohols, procaine and hyperthermia on the protein content of nuclei and chromatin.
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酒精、普鲁卡因和热疗对细胞核和染色质蛋白质含量的影响。
DOI：
10.1080/09553008414551031
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Wilson,CF
中科院分区：
文献类型：
--
作者：
RotiRoti,JL;Wilson,CF
由于以前的研究表明，热诱导的核变化与细胞杀伤相关，我们已经通过测量HeLa细胞中的核和染色质蛋白质含量来扩展这些观察结果，这些细胞被认为是引起膜损伤的热致敏剂。具体而言，我们研究了酒精（C2-C5）和普鲁卡因的影响。将HeLa细胞暴露于浓度大于1 M乙醇或0·2 M丁醇的酒精中30分钟，导致细胞核和染色质的蛋白质含量可测量地增加。当细胞在酒精存在下加热（45°C）时，细胞核和染色质蛋白质含量的增加显著大于浓度高于0.25 M乙醇和0.07 M丁醇的单独加热。此外，当细胞在45°C下加热0 - 60 min时，0.41 M乙醇的存在导致染色质吸收的蛋白质量增加2倍。普鲁卡因也观察到类似的效果。因此，单独的酒精或普鲁卡因可以引起染色质蛋白质含量的增加，并且可以与热协同作用以引起更大的增加。这些结果表明，膜损伤可能会导致染色质蛋白质含量增加，从而导致细胞死亡。
Since previous studies suggest that heat-induced nuclear changes correlate with cell killing we have extended these observations by measuring nuclear and chromatin protein content in HeLa cells heat sensitized by agents which are believed to cause membrane damage. Specifically, we have investigated the effects of alcohol (C2–C5) and procaine. Exposure of HeLa cells to alcohol concentrations greater than 1 M ethanol or 0·2 M butanol for 30 min causes a measurable increase in the protein content of both nuclei and chromatin. When cells were heated (45°C) in the presence of alcohol, the increase in nuclear and chromatin protein content was significantly greater than that for heat alone at concentrations above 0·25 M ethanol and 0·07 M butanol. In addition, the presence of 0·41 M ethanol causes a two-fold increase, over heat alone, in the amount of protein absorbed to chromatin when cells are heated at 45°C from 0 to 60 min. Similar effects were observed with procaine. Thus, alcohol or procaine alone can cause an increase in chromatin protein content and can act synergistically with heat to cause a larger increase. These results suggest that membrane damage may cause a larger increased protein content of chromatin and thereby lead to cell death.