On the reactivity of bromoperoxidase I (Ascophyllum nodosum) in buffered organic media: Formation of carbon bromine bonds
On the reactivity of bromoperoxidase I (Ascophyllum nodosum) in buffered organic media: Formation of carbon bromine bonds
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溴过氧化物酶 I（Ascophyllum nodosum）在缓冲有机介质中的反应性：碳溴键的形成
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
H. Vilter
中科院分区：
文献类型：
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作者：
J. Hartung;Y. Dumont;Marco Greb;Diana Hach;F. Köhler;H. Schulz;M. Časný;D. Rehder;H. Vilter
钒酸盐(V)依赖性溴过氧化物酶（BPO） I (Ascophyllum nodosum) [VBrPO(AnI)]在h2o2孵育、morpholin-4-乙烷磺酸（MES）缓冲的酒精水溶液中保存，其过氧化物酶（PO）活性保持在60天左右。这些条件适用于将溴和，例如，2-甲基吡咯-羧酸甲酯转化为溴吡咯，具有几乎定量的过氧化物产率。在磷酸盐缓冲、ch3cn稀释的介质中，用H2O2、KBr和VBrPO（AnI）处理后，δ，ε-不饱和醇生成β-溴丙烷和溴环化产物，即四氢呋喃和四氢吡喃（70 - 84%的质量平衡）。
Peroxidase (PO) activity of vanadate(V)-dependent bromoperoxidase (BPO) I (Ascophyllum nodosum) [VBrPO(AnI)] was retained with a half-life time of ~60 days, if stored in H2O2-incubated, morpholin-4-ethane sulfonic acid (MES)-buffered aqueous alcoholic solutions. These conditions were applied for converting bromide and, e.g., methyl pyrrole-2-carboxylate into bromopyrroles with an almost quantitative peroxide yield. δ,ε-unsaturated alcohols furnished β-bromohydrins and products of bromocyclization, i.e., tetrahydrofurans and tetrahydropyrans (70–84 % mass balance), if treated with H2O2, KBr, and VBrPO(AnI) in phosphate-buffered, CH3CN-diluted media.