Indocyanine Green-Loaded Silver Nanoparticle@Polyaniline Core/Shell Theranostic Nanocomposites for Photoacoustic/Near-Infrared Fluorescence Imaging-Guided and Single-Light-Triggered Photothermal and Photodynamic Therapy
Indocyanine Green-Loaded Silver Nanoparticle@Polyaniline Core/Shell Theranostic Nanocomposites for Photoacoustic/Near-Infrared Fluorescence Imaging-Guided and Single-Light-Triggered Photothermal and Photodynamic Therapy
复制标题
用于光声/近红外荧光成像引导和单光触发光热和光动力治疗的吲哚菁绿载银纳米粒子@聚苯胺核/壳治疗纳米复合材料
DOI：
10.1021/acsami.6b11262
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发表时间：
2016-12-28
影响因子：
9.5
通讯作者：
Li, Nan
中科院分区：
文献类型：
--
作者：
Tan, Xiaoxiao;Wang, Jinping;Li, Nan
近年来，光声/近红外荧光双模式成像引导光疗技术得到了广泛的研究。然而，开发高效、简化的治疗诊断系统来放大成像引导的光热疗法/光动力疗法（PTT/PDT）仍然是一个巨大的挑战。本文成功构建了一种单光触发的吲哚菁绿色（ICG）负载聚乙二醇化银纳米粒子核/聚苯胺壳（Ag@PANI）纳米复合材料（ICG-Ag@PANI），用于PA/NIRF成像引导增强PTT/PDT协同效应。在这项研究中，所合成的Ag@PANI纳米复合材料不仅被用作有前途的光热剂，而且还作为潜在的纳米载体，通过π-π堆积和疏水相互作用负载光敏剂ICG。所制备的ICG-Ag@PANI具有许多优越的上级性质，如在近红外（NIR）区域的强光吸收，增强的ICG光稳定性，以及出色的NIR激光诱导局部热疗和活性氧（ROS）产生。在体内研究中，PA/NIRF双模式成像证实了ICG-Ag@PANI通过增强的渗透性和保留（EPR）效应在肿瘤区域中的积累和分布。此外，ICG-Ag@PANI的PTT效应在5分钟内迅速将肿瘤温度升高至56.8 ℃。这也表明，ICG的细胞毒性ROS产生能力在负载到Ag@PANI纳米复合材料后得到了很好的保持。值得注意的是，与单独的PTT或PDT相比，单一808 nm NIR激光触发的组合PTT/PDT疗法在体外表现出增强的HeLa细胞致死率和在体内表现出增强的肿瘤生长抑制。
Photoacoustic (PA)/near-infrared fluorescence (NIRF) dual-modal imaging-guided phototherapy has been wide explored very recently. However, the development of high-efficiency and simplified-performed theranostic system for amplifying imaging-guided photothermal therapy/photodynamic therapy (PTT/PDT) is still a great challenge. Herein, a single-light-triggered indocyanine green (ICG)-loaded PEGylation silver nanoparticle core/polyaniline shell (Ag@PANI) nanocomposites (ICG-Ag@PANI) for PA/NIRF imaging-guided enhanced PTT/PDT synergistic effect has been successfully constructed. In this study, the synthesized Ag@PANI nanocomposites are utilized not only as the promising photothermal agent but also as potential nanovehicles for loading photosensitizer ICG via pi-pi stacking and hydrophobic interaction. The as-prepared ICG-Ag@PANI possesses many superior properties such as strong optical absorption in the near-infrared (NIR) region, enhanced photostability of ICG, as well as outstanding NIR laser-induced local hyperthermia and reactive oxygen species (ROS) generation. In the in vivo study, PA/NIRF dual-modal imaging confirms the accumulation and distribution of ICG-Ag@PANI in the tumor region via enhanced permeability and retention (EPR) effect. Moreover, the PTT effect of ICG-Ag@PANI rapidly raised the tumor temperature to 56.8 degrees C within 5 min. It is also demonstrated that the cytotoxic ROS generation ability of ICG is well maintained after being loaded onto Ag@PANI nanocomposites. Remarkably, in comparison with PTT or PDT alone, the single 808 nm NIR laser-triggered combined PTT/PDT therapy exhibits enhanced HeLa cells lethality in vitro and tumor growth inhibition in vivo.