Effect of ovariectomy on the local residual ridge remodeling.
Effect of ovariectomy on the local residual ridge remodeling.
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卵巢切除术对局部残余嵴重塑的影响。
DOI：
10.1016/s0022-3913(97)70169-0
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Nishimura,I
中科院分区：
文献类型：
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作者：
Jahangiri,L;Kim,A;Nishimura,I
骨质疏松症和缺牙症是两种影响美国老年人的疾病（分别为24和25百万）。这两种疾病是相互独立的，但是，他们有几个共同的病理症状，如减少骨量.PurposeThe本研究的目的是确定是否雌激素缺乏症或其替代疗法有任何影响的现象残留的脊remodeling.Material和methodsThree动物组形成，由6只雌性Sprague-Dawley大鼠。两组均行卵巢切除术，并接受溶剂溶液或通过渗透泵输送的每日剂量（1.5 μg/天）的17β-雌二醇。对照组进行假手术并接受溶剂溶液。在整个实验过程中，动物成对喂养。单侧磨牙拔除在上颌骨，这产生了一个合适的网站检查的组织学特征和分子生物学分析。在4周postextraction期间的骨重建活动，注意到在表面上的剩余的脊在control group.ResultsThe卵巢切除组表现出增加的骨吸收活动，而表面上的剩余的脊牙槽骨的卵巢切除和雌激素治疗组被覆盖的透明组织层。Poly（A）+核糖核酸样品从重塑残留牙槽嵴组织中分离。通过核糖核酸转移斑点印迹法检测α2（I）、α1（II）、α1（IX）和α1（X）胶原的表达。与对照组相比，卵巢切除动物显示骨形成减少，I型和II型胶原表达减少。相反，雌激素治疗组显示I型胶原蛋白的形成减少，II型胶原蛋白的表达增加。进一步检查II型胶原蛋白形成的卵巢切除和雌激素治疗组通过原位杂交揭示了显着的标记IIA型胶原蛋白探针，这是与表面组织的残留脊alloy bone.ConclusionThese研究结果表明，雌激素缺乏症及其替代治疗似乎影响残留的脊骨重塑的活动在分子水平上。（J Prosthet Dent 1997;77：435-43.）
Statement of problemOsteoporosis and edentulism are two disease processes that affect a large group of elderly people in the United States (24 and 25 million, respectively). These two diseases are independent of each other; however, they have several pathologic symptoms in common, such as reduction in bone mass.PurposeThe purpose of this study was to determine whether estrogen deficiency or its replacement therapy have any effect on the phenomenon of residual ridge remodeling.Material and methodsThree animal groups were formed that consisted of six female Sprague-Dawley rats each. The two groups had ovariectomy and received either a vehicle solution or a daily dose (1.5 μg/day) of 17β-estradiol delivered through osmotic pumps. The control group underwent sham surgery and received a vehicle solution. Animals were pair fed throughout the experiment. Unilateral molar extraction was performed in the maxilla, which produced a suitable site for examination of histologic characteristics and molecular biologic analyses. At the 4-week postextraction period the bone remodeling activity was noted at the surface of the residual ridge in the control group.ResultsThe ovariectomized group showed increased bone resorption activity, whereas the surface of the residual ridge alveolar bone of the ovariectomized and estrogen-treated group was covered by a layer of hyaline tissue. Poly(A)+ ribonucleic acid samples were isolated from the remodeling residual ridge tissues. Expression of α2(I), α1(II), α1(IX), and α1(X) collagens were examined by ribonucleic acid transfer dot blots. Compared with the control group, ovariectomized animals showed a reduction in bone formation with decreased expressions of type I and II collagens. In contrast, the estrogen-treatment group showed decreased formation of type I collagen with a much increased expression of type II collagen. Further examination of type II collagen formation on the ovariectomized and estrogen-treated group by means of in situ hybridization revealed the notable labeling by the type IIA collagen probe, which was associated with the surface tissue of the residual ridge alveolar bone.ConclusionThese findings suggest that estrogen deficiency and its replacement therapy seem to affect the activity of residual ridge bone remodeling at the molecular level. (J Prosthet Dent 1997;77:435-43.)