SOME FACTORS FOR TUBERIZATION IN THE POTATO PLANT

SOME FACTORS FOR TUBERIZATION IN THE POTATO PLANT
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DOI:
10.2307/2438945
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发表时间:
1956-01-01
影响因子:
3
通讯作者:
GREGORY, LE
GREGORY, LE
中科院分区:
生物学3区
文献类型:
--
作者:
GREGORY, LE

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马铃薯植物 (Solanum tuberosum L.) var Kennebec 中的块茎萌生似乎是通过特定温度和光周期条件形成或激活的嫁接传播刺激来促进的。事实证明,即使将植物移至不利于块茎形成的条件下,这种刺激仍然持续存在。当植物在20℃下保持8小时时,实现了丰富的块茎形成。日光照射后,在 14℃ 下进行 16 小时的黑暗期。 (感应环境)。当植物在26℃下保存8小时时没有形成块茎。日光,然后是 8 小时。人造光(1000f.-c.),20℃,8小时。 20°C 黑暗(无感应环境)。如果来自诱导植物,则叶插条种植在蛭石中时,在种植后7至14天在叶腋处形成块茎,但如果从非诱导环境下生长的植物切下插条,则不会形成块茎。来自用作供体的诱导马铃薯植物的叶芽,当嫁接物与作为受体的非诱导插条结合时,导致后者在非诱导条件下种植嫁接插条7天后结块。茎片2厘米。长有腋芽的用0.3%氯化汞消毒1分钟。随后 5 分钟。 10% Chlorox 中,并在无菌培养物中生长。当诱导植物的茎片在含 1% 琼脂的 3% 蔗糖培养基和含 3% 蔗糖加无机营养物的 1% 琼脂培养基中生长时,在腋芽处形成块茎。 1%琼脂中没有形成块茎。当将非诱导植物的茎块种植在含 3%、5%、10% 蔗糖的 1% 琼脂培养基中时,从腋芽中发育出芽。当种植在1%琼脂和20%蔗糖的1%琼脂培养基中时,没有发生芽生长。
Tuber initiation in the potato plant (Solanum tuberosum L.) var Kennebec, appears to be promoted by a graft transmissible stimulus formed or activated by specific conditions of temperature and photoperiod. The stimulus proved to be persistent even after moving the plants to conditions unfavorable for tuber formation. Profuse tuber formation was achieved when the plants were kept at 20[degree]C during 8 hr. of daylight followed by a dark period at 14[degree]C for 16 hr. (inductive environment). No tubers formed when the plants were kept at 26[degree]C for 8 hr. of day-light, followed by 8 hr. of artificial light (1000f.-c.) at 20[degree]C, and 8 hr. of darkness at 20[degree]C (non-inductive environment). Leafy cuttings when planted in vermiculite developed tubers at the leaf axils, 7 to 14 days after planting if coming from induced plants, but no tubers formed if the cuttings were excised from plants growing under non-inducing environment. A leafy shoot from an induced potato plant used as donor, when graft united to a non-induced cutting as receptor caused the latter to tuberize seven days after planting the grafted cutting under non-inducing con-ditions. Stem pieces 2 cm. long with an axillary bud were dis-infected with mercuric chloride, 0.3% for 1 min. followed by 5 min. in 10% Chlorox, and grown in sterile culture. Stem pieces from induced plants formed tubers at the axillary bud when grown in 3% sucrose in 1% agar medium, and in 3% sucrose plus inorganic nutrients in 1% agar. No tubers formed in 1% agar. Stem pieces from non-induced plants developed shoots from the axillary bud when planted in 3%, 5%, 10% sucrose in 1% agar medium. No shoot growth took place when planted in 1% agar, and 20% sucrose in 1% agar medium.