Dynamic formation of massive micro-bead arrays using hybrid optical tweezers
Dynamic formation of massive micro-bead arrays using hybrid optical tweezers
复制标题
使用混合光镊动态形成大量微珠阵列
DOI：
10.1007/978-3-642-23508-5_332
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
H. Kitajima
中科院分区：
文献类型：
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作者：
Y. Tanaka;S. Tsutsui;M. Ishikawa;H. Kitajima
动态微阵列具有很大的灵活性和潜力的传感仪器，在诊断和生物医学领域的先进研究。与静态微阵列（例如使用生物分子的微点的DNA芯片）相比，动态微阵列使用移动的基质，通常是涂覆有生物分子的微/纳米珠。为了实现动态微阵列，先进的珠处理技术是必不可少的，允许运输选定的珠和它们的固定信号检测。多光束光镊的使用是用于布置和处理众多微/纳米珠的最合适的技术之一。对于大量的微珠阵列的动态形成，我们已经开发了一个混合系统，包括使用两个光镊，GPC方法和机械扫描方法，使用一个单一的激光束源的技术。本文给出了4个典型的大质量动力学阵列的实例：1）10×10微珠阵列的组装及其单元的处理，2）7×7阵列的放大、缩小和旋转，3）双色7×7阵列的组装，4）两组4×4阵列的组装。我们还描述了混合光镊系统的配置。
Dynamic microarrays have great flexibility and potential as sensing instruments for advanced research in diagnostics and biomedical fields. In contrast with static microarrays, such as DNA-chips using micro-spots of biomolecules, dynamic microarrays use mobile substrata, usually micro/nano-beads coated with bio-molecules. To realize dynamic microarrays, advanced bead handling techniques are essential, allowing for the transportation of selected beads and their immobilization for signal detection. The use of multibeam optical tweezers is one of the most suitable techniques for the arranging and handling of numerous micro/nanobeads. For the dynamic formation of massive micro-bead arrays, we have developed a hybrid system consisting of techniques using two optical tweezers, the GPC method and a mechanical scanning method, using a single laser beam source. In the present paper, four typical examples of massive dynamical arrays are demonstrated: 1) the assembly of a 10×10 micro-bead array and the handling of its elements, 2) the enlargement, shrinking and rotation of a 7×7 array, 3) the assembly of a two-colored 7×7 array, and 4) the assembly of two sets of a 4×4 array. We also describe the configuration of the hybrid optical tweezers system.