In vivo imaging of the mouse neurovascular unit under chronic cerebral hypoperfusion.
In vivo imaging of the mouse neurovascular unit under chronic cerebral hypoperfusion.
复制标题
慢性脑灌注不足下小鼠神经血管单元的体内成像。
DOI：
10.1161/strokeaha.114.005891
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Tomimoto H.
中科院分区：
文献类型：
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作者：
Yata K;Nishimura Y;Unekawa M;Tomita Y;Suzuki N;Tanaka T;Mizoguchi A;Tomimoto H.
背景和目的正常的脑功能是由一个称为神经血管单位（NVU）的完整系统维持的，该系统由细胞和非细胞成分组成。虽然特定的神经血管成分的个体特征已被了解，但它们作为一个功能单位如何对缺血应激作出反应尚不清楚。因此，我们建立了一种在体成像方法，并澄清了NVU对慢性脑低perfusion.MethodsGreen小鼠（b-act-EGFP）与SR 101血浆标记在本实验中使用。在左侧躯体感觉皮层上制作一个闭合的颅骨窗。为了模拟慢性脑灌注不足，使用微线圈对小鼠进行双侧颈总动脉狭窄手术。在体内实时成像进行使用2-光子激光扫描显微镜在术前期间，和后1天和1和2周的双侧颈总动脉狭窄或sham operations.ResultsOur方法允许三维观察的NVU的大部分组成部分，以及动态毛细血管微循环。在慢性脑灌注不足的情况下，我们没有检测到NVU中每个细胞成分的任何结构变化;然而，在很长一段时间内检测到微循环障碍。软脑膜小动、静脉内可见白细胞滚动和粘附，后者更为明显。在深层皮质毛细血管，流动停滞，因为白细胞堵塞经常observed.ConclusionsWe建立了一个实时可视化的NVU在体内成像方法。提示慢性脑低灌注状态下，白细胞活化在微循环障碍中起重要作用。
Background and PurposeProper brain function is maintained by an integrated system called the neurovascular unit (NVU) comprised cellular and acellular elements. Although the individual features of specific neurovascular components are understood, it is unknown how they respond to ischemic stress as a functional unit. Therefore, we established an in vivo imaging method and clarified the NVU response to chronic cerebral hypoperfusion.MethodsGreen mice (b-act-EGFP) with SR101 plasma labeling were used in this experiment. A closed cranial window was made over the left somatosensory cortex. To mimic chronic cerebral hypoperfusion, mice were subjected to bilateral common carotid artery stenosis operations using microcoils. In vivo real-time imaging was performed using 2-photon laser-scanning microscopy during the preoperative period, and after 1 day and 1 and 2 weeks of bilateral common carotid artery stenosis or sham operations.ResultsOur method allowed 3-dimensional observation of most of the components of the NVU, as well as dynamic capillary microcirculation. Under chronic cerebral hypoperfusion, we did not detect any structural changes of each cellular component in the NVU; however, impairment of microcirculation was detected over a prolonged period. In the pial small arteries and veins, rolling and adhesion of leukocyte were detected, more prominently in the latter. In the deep cortical capillaries, flow stagnation because of leukocyte plugging was frequently observed.ConclusionsWe established an in vivo imaging method for real-time visualization of the NVU. It seems that under chronic cerebral hypoperfusion, leukocyte activation has a critical role in microcirculation disturbance.