Reconstruction of Critical-Sized Bone Defect in Dog Skull by Octacalcium Phosphate Combined with Collagen
Reconstruction of Critical-Sized Bone Defect in Dog Skull by Octacalcium Phosphate Combined with Collagen
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DOI：
10.1111/j.1708-8208.2009.00192.x
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发表时间：
2011-01-01
影响因子：
3.6
通讯作者：
Echigo, Seishi
中科院分区：
文献类型：
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作者：
Kawai, Tadashi;Matsui, Keiko;Echigo, Seishi
目的：探讨人工合成磷酸八钙(OCP)复合胶原(OCP/胶原)能否修复犬颅骨临界大小的缺损。OCP/胶原蛋白可被生物降解，如果植入大鼠颅骨缺损处，有可能被新形成的骨替代。材料和方法：从猪真皮和人工合成的OCP中分离出胃酶消化的成胶原蛋白，制备OCP/胶原蛋白复合片。在一只狗的头骨上制造了两个临界大小的缺陷(直径20 mm)。于植入后3、6、12个月，将10个OCP/胶原或胶原(对照)圆盘植入骨缺损区，与周围组织一起切除。结果：X射线衍射和傅立叶变换红外光谱分析表明，OCP在3个月后有向低结晶羟基磷灰石转变的趋势，与生物磷灰石相似。放射学和组织学分析显示，植入OCP/胶原盘后3个月，缺损区开始形成新骨。然而，即使长达12个月的植入，对照胶原盘也没有促进骨形成。组织形态计量学分析显示，植入OCP/胶原的缺损区新骨形成百分率显著增加，从3个月的30.91+/-6.65增加到12个月的51.22+/-5.99，但在6个月时趋于平台值(44.49+/-3.34)。另一方面，3个月时OCP的残留率估计在10%左右，此后几乎没有变化。结论：OCP/胶原可促进犬颅骨临界大小缺损区的骨再生3~6个月，OCP/胶原具有潜在的骨替代材料的潜力。
Purpose:The present study was designed to investigate whether synthetic octacalcium phosphate (OCP) combined with collagen (OCP/collagen) can repair a critical-sized defect in dog skull. OCP/collagen has been shown to biodegrade and to tend to be replaced by newly formed bone if implanted in rat calvaria defects.Materials and Methods:An OCP/collagen disk was prepared from pepsin-digested atelocollagen isolated from porcine dermis and synthetic OCP. Two critical-sized defects (20 mm in diameter) were made in a dog skull. Ten disks of OCP/collagen or collagen (control) were implanted in the bone defects and resected with surrounding tissues at 3, 6, or 12 months after the implantation. The specimens were analyzed radiographically, crystallographically, histologically, and histomorphometrically.Results:X-ray diffraction and FTIR analyses showed that OCP tended to convert to a poorly crystallized hydroxyapatite, similar to that of biological apatite, by 3 months. Radiographic and histologic analyses showed that the implantation of OCP/collagen disks initiated new bone formation in the defects at 3 months after implantation. However, there was no promotion of bone formation by control collagen disks even with prolonged implantation up to 12 months. Histomorphometric analysis revealed that the percentage of newly formed bone in the defect implanted with OCP/collagen increased significantly, from 30.91 +/- 6.65 at 3 months to 51.22 +/- 5.99 at 12 months, although the value tended to reach a plateau at 6 months (44.49 +/- 3.34). On the other hand, the percentage of remaining OCP was estimated at approximately 10% at 3 months and remained nearly unchanged thereafter.Conclusion:The results suggest that bone regeneration of a critical-sized bone defect of dog calvaria by OCP/collagen can be enhanced for 3 to 6 months and that OCP/collagen holds potential as a bone substitute material.