THE ACTIVITIES OF 2 ETS-RELATED TRANSCRIPTION FACTORS REQUIRED FOR DROSOPHILA EYE DEVELOPMENT ARE MODULATED BY THE RAS/MAPK PATHWAY
THE ACTIVITIES OF 2 ETS-RELATED TRANSCRIPTION FACTORS REQUIRED FOR DROSOPHILA EYE DEVELOPMENT ARE MODULATED BY THE RAS/MAPK PATHWAY
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DOI：
10.1016/0092-8674(94)90580-0
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发表时间：
1994-07-15
期刊：
影响因子：
64.5
通讯作者：
RUBIN, GM
中科院分区：
文献类型：
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作者：
ONEILL, EM;REBAY, I;RUBIN, GM
我们发现果蝇正常眼睛发育所需的两种ets相关转录因子（尖和眼）的活性受到Ras1/MAPK通路的调控。该尖基因编码两种相关蛋白，我们发现其中一种形式是转录的组成激活因子，而另一种形式的活性是由Ras1/MAPK途径刺激的。第二种形式的单一共识MARK磷酸化位点的突变消除了这种反应性。yan是光感受器决定的负调节因子，遗传数据表明它可以作为Ras1的拮抗剂。我们证明yan可以抑制转录，并且这种抑制活性受到Ras1/MAPK信号的负调控，最有可能是通过MAPK直接磷酸化yan。
We show that the activities of two Ets-related transcription factors required for normal eye development in Drosophila, pointed and yan, are regulated by the Ras1/MAPK pathway. The pointed gene codes for two related proteins, and we show that one form is a constitutive activator of transcription, while the activity of the other form is stimulated by the Ras1/MAPK pathway. Mutation of the single consensus MARK phosphorylation site in the second form abrogates this responsiveness. yan is a negative regulator of photoreceptor determination, and genetic data suggest that it acts as an antagonist of Ras1. We demonstrate that yan can repress transcription and that this repression activity is negatively regulated by the Ras1/MAPK signal, most likely through direct phosphorylation of yan by MAPK.