RIBOCHARIN - A NUCLEAR MR 40,000 PROTEIN-SPECIFIC TO PRECURSOR PARTICLES OF THE LARGE RIBOSOMAL-SUBUNIT
RIBOCHARIN - A NUCLEAR MR 40,000 PROTEIN-SPECIFIC TO PRECURSOR PARTICLES OF THE LARGE RIBOSOMAL-SUBUNIT
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DOI：
10.1016/s0092-8674(85)80034-9
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
FRANKE, WW
中科院分区：
文献类型：
--
作者：
HUGLE, B;SCHEER, U;FRANKE, WW
用单抗(No-194)在非洲爪哇和其他两栖动物中鉴定了一种核糖蛋白，它与核仁和核质65S颗粒的颗粒成分有特异性的联系。这些颗粒含有核28S rRNA，显然是核质运输中大核糖体亚单位的前体。免疫电子显微镜下核糖蛋白定位于核仁颗粒成分和间染色质颗粒中。在有丝分裂过程中，含有核糖素的颗粒与染色体表面结合，并在末期晚期的重组核仁中重新聚集。核糖精可能是大核糖体亚单位前体颗粒的特定成分，在穿过核膜之前从65S颗粒中解离出来，并在核糖体生物发生中重新利用。
A monoclonal antibody (No-194) was used to identify, in Xenopus laevis and other amphibia, an acidic protein of MW 40,000 (ribocharin) which is specifically associated with the granular component of the nucleolus and nucleoplasmic 65S particles. These particles contain the nuclear 28S rRNA and apparently represent the precursor to the large ribosomal subunit in nucleocytoplasmic transit. By immunoelectron microscopy ribocharin was localized in the granular component of the nucleolus and in interchromatin granules. During mitosis ribocharin-containing particles are associated with surfaces of chromosomes and are recollected in the reconstituting nucleoli in late telophase. Ribocharin may be a specific component of precursor particles of the large ribosomal subunit, which dissociates from the 65S particle before passage through the nuclear envelope, and is reutilized in ribosome biogenesis.