BIR2 affects complex formation of BAK1 with ligand binding receptors in plant defense
BIR2 affects complex formation of BAK1 with ligand binding receptors in plant defense
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DOI：
10.4161/psb.28944
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发表时间：
2014-01-01
影响因子：
2.9
通讯作者：
Kemmerling, Birgit
中科院分区：
文献类型：
--
作者：
Halter, Thierry;Imkampe, Julia;Kemmerling, Birgit
BAK1是一种多功能的富含亮氨酸的重复受体激酶（LRR-RLK），它通过与多种配体结合受体相互作用来发挥其功能，从而影响从通过PAMP和DAMP感知油菜素类固醇到细胞死亡控制的多种过程。我们最近发现了一个新的BAK1相互作用蛋白BIR2，它也是一个LRR-RLK。与BAK1相反，BIR2负调控BAK1依赖性PAMP反应。虽然油菜素类固醇反应不受BIR2的影响，但细胞死亡受到BAK1的负调控。配体感知后，BIR2从BAK1中释放出来，增加了可与活化的配体结合受体形成复合物的自由BAK1库。单个配体只能从BIR2中部分释放BAK1。暴露于配体混合物后，几乎可以释放全部的BAK1，这表明BAK1与BIR2一起存在于可被特定配体单独定位的子池中。这些数据支持BAK1存在于与其配体结合受体伙伴的预形成复合物中的观点。BIR2过表达导致BAK1与FLS2复合物形成减少，表明BIR2负性调节BAK1与配体结合受体复合物的形成。
BAK1 is a multifunctional leucine-rich repeat receptor kinase (LRR-RLK) that exerts its function by interacting with multiple ligand binding receptors and thereby influences diverse processes varying from brassinosteroid perception via PAMP and DAMP perception to cell death control. We recently identified a new BAK1 interacting protein, BIR2, that is also a LRR-RLK. In contrast to BAK1, BIR2 negatively regulates BAK1-dependent PAMP responses. While brassinosteroid responses are not affected by BIR2, cell death is negatively regulated as described for BAK1. BIR2 is released from BAK1 after ligand perception, increasing the pool of free BAK1 that is available to form complexes with activated ligand binding receptors. Individual ligands can only partially release BAK1 from BIR2. After exposition to a cocktail of ligands, almost the complete amount of BAK1 can be released indicating that BAK1 exists, together with BIR2, in subpools that can be individually addressed by specific ligands. These data support the idea that BAK1 exists in preformed complexes with its ligand binding receptor partners. Overexpression of BIR2 results in reduced complex formation of BAK1 with FLS2, showing that BIR2 negatively regulates BAK1 complex formation with ligand binding receptors.