Automatic quantitative analysis of t-tubule organization in cardiac myocytes using ImageJ
Automatic quantitative analysis of t-tubule organization in cardiac myocytes using ImageJ
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DOI：
10.1152/ajpcell.00259.2014
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发表时间：
2015-02-01
影响因子：
5.5
通讯作者：
Maupoil, Veronique
中科院分区：
文献类型：
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作者：
Pasqualin, Come;Gannier, Francois;Maupoil, Veronique
横小管系统在哺乳动物横纹肌是高度有组织的，有助于最佳和均匀的收缩。心衰和房颤等多种病理包括t小管紊乱和收缩功能障碍。很少有工具可用于量化t型管系统的组织。我们为国家卫生研究院开发的ImageJ/Fiji图像分析平台开发了一个插件。这个插件（TTorg）分析原始共聚焦显微镜图像。分析选项包括整个图像、图像的特定区域（裁剪）和同一图像的z轴分析。对具有相同标准的一系列图像进行批量分析也是一种选择。不需要将任何标本重新定向到水平方向，也不需要对图像进行阈值处理来进行分析。TTorg包含一个合成的“mycell -like”图像生成器，用于在用户自己的实验条件下测试插件的效率。该插件在不同模拟细胞特性和采集参数的合成图像上进行了验证。从野生型和肌营养不良小鼠分离的小鼠心室肌细胞的实验数据中，TTorg能够检测到t小管系统组织的显著差异。TTorg是免费发布的，它的源代码是可用的。它在各种实验条件下提供了可靠，易于使用，自动和无偏的t管组织测量。
The transverse tubule system in mammalian striated muscle is highly organized and contributes to optimal and homogeneous contraction. Diverse pathologies such as heart failure and atrial fibrillation include disorganization of t-tubules and contractile dysfunction. Few tools are available for the quantification of the organization of the t-tubule system. We developed a plugin for the ImageJ/Fiji image analysis platform developed by the National Institutes of Health. This plugin (TTorg) analyzes raw confocal microscopy images. Analysis options include the whole image, specific regions of the image (cropping), and z-axis analysis of the same image. Batch analysis of a series of images with identical criteria is also one of the options. There is no need to either reorientate any specimen to the horizontal or to do a thresholding of the image to perform analysis. TTorg includes a synthetic "myocyte-like" image generator to test the plugin's efficiency in the user's own experimental conditions. This plugin was validated on synthetic images for different simulated cell characteristics and acquisition parameters. TTorg was able to detect significant differences between the organization of the t-tubule systems in experimental data of mouse ventricular myocytes isolated from wild-type and dystrophin-deficient mice. TTorg is freely distributed, and its source code is available. It provides a reliable, easy-to-use, automatic, and unbiased measurement of t-tubule organization in a wide variety of experimental conditions.