Public perception about drinking jar water and its bacteriological analysis.

Public perception about drinking jar water and its bacteriological analysis.
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公众对饮用罐装水的看法及其细菌学分析。

DOI:
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发表时间:
2010
期刊:
Nepal Medical College journal : NMCJ
影响因子:
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通讯作者:
M. Aryal
M. Aryal
中科院分区:
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文献类型:
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作者:
M. Subedi;M. Aryal

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这些天来,瓶装水的消耗量一直在不断增加。为了改善这种水质和供水,需要有资料来评估各种社区的水污染情况,包括那些主要依赖未经改善的分散式饮用水源的社区。这项研究是为了评估公众对饮用罐水的看法和评估分布在加德满都山谷的饮用罐水,这是在微生物学系,阿姆利特科学校园,Thamel,加德满都在2009年8月至2009年12月期间进行的。使用标准方案处理具有不同品牌名称的不同饮用罐水的总共57个水样,并分析总大肠菌群和粪大肠菌群的存在。采用K-B纸片扩散法对不同水样中分离的粪大肠菌群进行体外药敏试验。为了解人们对饮用桶装水的看法,采用半结构式问卷调查方法,对525名饮用桶装水的人群进行了调查。大多数人认为他们的饮用罐水好,但发现高度关注的质量。总大肠菌群检出率为91.2%(n=52),粪大肠菌群检出率为59.6%。在研究期间,分离出117株肠道细菌,其中33.3%(n=39)为大肠埃希菌,其次为其他革兰氏阴性菌。在58株粪大肠菌群中,大肠埃希菌分别占43.1%、39.6%、12.2%;大肠杆菌、克雷伯氏菌属和产气肠杆菌。这些粪大肠菌群,都是敏感的抗生素环丙沙星和耐氨苄青霉素。这一发现表明,缸水是不安全的饮用目的,未经处理。
The consumption of jar water has been increasing consistently in these days. To improve such water quality and supply, information is needed to assess water contamination in a variety of community, including those that rely primarily on unimproved distributed sources of drinking water. This study was done to assess the public perception on drinking jar water and assessment of drinking jar water distributed in Kathmandu Valley which was conducted in the Department of Microbiology, Amrit Science Campus, Thamel, Kathmandu during the period of Aug 2009 to Dec 2009. A total 57 water samples of different drinking jar water having different brand names were proceed using standard protocols and analyzed for the presence of total coliforms and fecal coliforms. All identified fecal coliforms isolates from different water samples were subjected to in-vitro antimicrobial susceptibility test by Kirby-Bauer disc diffusion method. In order to know the perception of people in drinking jar water, semi structured questionnaires were made and purposively selected for the study in 525 populations who were using jar water for drinking purpose. Most population rated their drinking jar water good but found to be highly concerned with the quality. Among total water samples, 91.2% (n=52) were found contaminated with total coliforms and 59.6% were with fecal coliforms. During the study, 117 isolates of enteric bacteria were isolated, of which 33.3% (n=39) were Escherichia coli followed by other gram negative bacteria. Similarly, out of 58 fecal coliforms isolates, 43.1%, 39.6%, 12.2% were E. coli, Klebsiella spp, and Enterobacter aerogens. Of those fecal coliforms, all were sensitive to antibiotic ciprofloxacin and resistant to ampicilin. The finding indicates that jar water is not safe for drinking purpose without treatment.