MAPPING OF T-CELL EPITOPES USING RECOMBINANT ANTIGENS AND SYNTHETIC PEPTIDES
MAPPING OF T-CELL EPITOPES USING RECOMBINANT ANTIGENS AND SYNTHETIC PEPTIDES
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DOI：
10.1002/j.1460-2075.1987.tb02360.x
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发表时间：
1987-05-01
期刊：
影响因子：
11.4
通讯作者：
YOUNG, DB
中科院分区：
文献类型：
--
作者：
LAMB, JR;IVANYI, J;YOUNG, DB
用两种互补的方法测定与麻风分枝杆菌65-kd抗原反应的克隆性和多克隆性人T淋巴细胞的表位特异性。用65-kd基因的部分片段构建的重组DNA亚库用于定位氨基酸序列101-146和409-526中的T细胞决定簇。独立地，蛋白质中潜在的T细胞表位是基于对氨基酸序列中特定模式的经验分析而预测的。在预测并随后合成的六个肽中，有两个(112-132和437-459)被证明包含人类T细胞表位。这证实和完善了利用重组DNA亚库获得的结果。这两个区域在麻风分枝杆菌和结核分枝杆菌中是相同的，与已知的65-kd蛋白的B细胞表位不同。重组DNA技术和多肽化学的结合在分析感染性病原体的细胞免疫反应方面可能被证明是有价值的。
Two complementary approaches were used to determine the epitope specificity of clonal and polyclonal human T lymphocytes reactive with the 65-kd antigen of Mycobacterium leprae. A recombinant DNA sublibrary constructed from portions of the 65-kd gene was used to map T cell determinants within amino acid sequences 101-146 and 409-526. Independently, potentail T cell epitopes within the protein were predicted based on an empirical analysis of specific patterns in the amino acid sequence. Of six peptides that were predicted and subsequently synthesized, two (112-132 and 437-459) were shown to contain human T cell epitopes. This corroborated and refined the results obtained using the recombinant DNA sublibrary. Both of these regions are identical in M. leprae and M. tuberculosis and are distinct from the known B cell epitopes of the 65-kd protein. This combination of recombinant DNA technology and peptide chemistry may prove valuable in analysis of the cellular immune response to infectious agents.