A recessive gene controlling male sterility sensitive to short daylength/low temperature in wheat (Triticum aestivum L.)
A recessive gene controlling male sterility sensitive to short daylength/low temperature in wheat (Triticum aestivum L.)
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DOI：
10.1631/jzus.b1000371
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发表时间：
2011-11
期刊：
影响因子：
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通讯作者：
Xiaodong Chen;Dong-fa Sun;D. Rong;Jun-hua Peng;Chengdao Li
中科院分区：
文献类型：
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作者：
Xiaodong Chen;Dong-fa Sun;D. Rong;Jun-hua Peng;Chengdao Li
利用光温敏核不育两系育种系统进行小麦杂交制种具有很大的潜力。337S是一个对短日照/低温和长日照/高温敏感的小麦雄性不育系。利用337S与5个普通小麦品种杂交获得的5个F2群体进行遗传分析。在短日照/低温条件下，所有F1‘S都是可育的，但F2代群体出现分离，育性比为3：1不育。结果表明，337S的雄性不育由1对隐性基因控制，暂定名为wptms3。采用混合分离分析(BSA)和简单序列重复(SSR)标记相结合的方法，利用一个作图群体定位不育基因。将wptms3基因定位在1BS染色体臂上，分别位于Xgwm413和Xgwm182两侧，遗传距离分别为3.2和23.5 cM。对标记辅助选择的准确性和效率进行了评估，证明了标记辅助选择对鉴定wptms3基因F2代隐性不育纯合子的准确性和效率是必要的。
Utilization of a two-line breeding system via photoperiod-thermo sensitive male sterility has a great potential for hybrid production in wheat (Triticum aestivum L.). 337S is a novel wheat male sterile line sensitive to both short daylength/low temperature and long daylength/high temperature. Five F2 populations derived from the crosses between 337S and five common wheat varieties were developed for genetic analysis. All F1’s were highly fertile while segregation occurred in the F2 populations with a ratio of 3 fertile:1 sterile under short daylength/low temperature. It is shown that male sterility in 337S was controlled by a single recessive gene, temporarily designated as wptms3. Bulked segregant analysis (BSA) coupled with simple sequence repeat (SSR) markers was applied to map the sterile gene using one mapping population. The wptms3 gene was mapped to chromosome arm 1BS and flanked by Xgwm413 and Xgwm182 at a genetic distance of 3.2 and 23.5 cM, respectively. The accuracy and efficiency of marker-assisted selection were evaluated and proved essential for identifying homozygous recessive male sterile genotypes of the wptms3 gene in F2 generation.