Amorphous carbon modification on implant surface: a general strategy to enhance osteogenic differentiation for diverse biomaterials via FAK/ERK1/2 signaling pathways
Amorphous carbon modification on implant surface: a general strategy to enhance osteogenic differentiation for diverse biomaterials via FAK/ERK1/2 signaling pathways
复制标题
种植体表面无定形碳修饰：通过 FAK/ERK1/2 信号通路增强多种生物材料成骨分化的一般策略
DOI：
10.1039/c8tb02850h
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发表时间：
2019
影响因子：
7
通讯作者：
Steve Guofang Shen
中科院分区：
文献类型：
--
作者：
Xinran Zhang;Haotian Li;Jiaqiang Liu;Hui Wang;Wenjun Sun;Kaili Lin;Xudong Wang;Steve Guofang Shen
骨移植在骨修复中起着至关重要的作用。然而，骨诱导能力低和成骨分化促进骨再生是骨移植的缺点。因此，开发一种通用、简便的技术来促进现有种植体的生物活性是势在必行的。在此，开发了一种简便的非晶态碳涂层方法，通过磁控溅射沉积在不同的生物材料上刺激成骨，包括生物陶瓷、生物金属和生物聚合物。结果表明，非晶态碳涂层修饰表面能显著促进骨髓间充质干细胞在各种生物材料表面的成骨。此外，还证实了FAK/ERK1/2信号通路参与了这种无定形碳涂层的成骨作用。大鼠颅骨缺损模型的骨再生能力证实了非晶态碳涂层诱导了更快的骨形成和矿化，提示非晶态碳涂层在体内具有促进成骨的作用。这些结果表明，用无定形碳修饰表面的方法为促进不同生物材料的成骨提供了一种普遍而简单的策略，这在骨修复应用中具有广阔的应用前景。
Bone implants play a crucial role in bone repairing. Nevertheless, low capability of osteoinductivity and osteogenic differentiation for bone regeneration are disadvantages of bone implants. Therefore, it is imperative to develop a general and facile technology to promote the bioactivity of existing implants. Herein, a facile amorphous carbon-coating approach was developed to stimulate osteogenesis on diverse biomaterials, including bioceramics, biometals, and biopolymers via magnetron sputtering deposition. The results confirmed that the amorphous carbon-coating-modified surfaces could significantly enhance osteogenesis of bone marrow mesenchymal stem cells (BMSCs) on every kind of biomaterial surface. Furthermore, it was demonstrated that the FAK/ERK1/2 signaling pathways were involved in the osteogenic effects of this amorphous carbon coating. The bone regeneration ability using the calvarial bone defect model of rats confirmed that the amorphous carbon coating induced faster bone formation and mineralization, which suggested the effect of amorphous carbon coating on stimulating osteogenesis in vivo. These results suggest that the approach involving modifying a surface with amorphous carbon provides a general and simple strategy to enhance the osteogenesis for diverse biomaterials, and this has promising potential for bone repairing applications.