Gene expression profiling of potato responses to cold, heat, and salt stress
Gene expression profiling of potato responses to cold, heat, and salt stress
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DOI：
10.1007/s10142-005-0141-6
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发表时间：
2005-10-01
影响因子：
2.9
通讯作者：
Buell, C. Robin
中科院分区：
文献类型：
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作者：
Rensink, Willem Albert;Iobst, Stacey;Buell, C. Robin
为了鉴定参与马铃薯非生物胁迫反应的基因，幼苗在受控条件下生长，并经受冷（4℃）、热（35℃）或盐（100 mM NaCl）胁迫长达27小时。使用类似于 12,000 个克隆马铃薯 cDNA 微阵列，在胁迫开始后的三个时间点（3、9 和 27 小时）从两个不同的组织、根和叶捕获表达谱。共有 3,314 个克隆被鉴定为对至少一种应激条件的反应显着上调或下调。这些克隆代表的基因编码转录因子、信号转导因子和热休克蛋白，这些蛋白与拟南芥和水稻的非生物胁迫反应相关，表明马铃薯中也有类似的反应途径。这些应激调节克隆可以分为应激特异性克隆或共享反应克隆，这表明存在一般反应途径以及更多应激特异性途径。此外，我们还确定了表达谱，这些表达谱表明施加于植物的胁迫类型。
In order to identify genes involved in abiotic stress responses in potato, seedlings were grown under controlled conditions and subjected to cold (4 degrees C), heat (35 degrees C), or salt (100 mM NaCl) stress for up to 27 h. Using an similar to 12,000 clone potato cDNA microarray, expression profiles were captured at three time points following initiation of the stress (3, 9, and 27 h) from two different tissues, roots and leaves. A total of 3,314 clones could be identified as significantly up- or down-regulated in response to at least one stress condition. The genes represented by these clones encode transcription factors, signal transduction factors, and heat-shock proteins which have been associated with abiotic stress responses in Arabidopsis and rice, suggesting similar response pathways function in potato. These stress-regulated clones could be separated into either stress-specific or shared-response clones, suggesting the existence of general response pathways as well as more stress-specific pathways. In addition, we identified expression profiles which are indicative for the type of stress applied to the plants.